Name | N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin |
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Synonyms |
4-[(2-acetamido-3-carboxypropanoyl)amino]-5-[[1-[[3-carboxy-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopropan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-5-oxopentanoic acid
Ac-DEVD-AMC MFCD00671411 L-α-Asparagine, N-acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)- N-Acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-(4-methyl-2-oxo-2H-chromen-7-yl)-L-α-asparagine |
Description | Ac-DEVD-AMC is the Caspase-3 substrate. Sequence: N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Methylcoumarin. |
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Related Catalog | |
In Vitro | Ac-DEVD-AMC is the caspase-3 substrate and could be used to monitor intracellular caspase-3 activity[1].The AMC moiety is highest at 2 h (area =5.6±1.8), the AMC peak area for Ac-DEVD-AMC incubated without lung specimen (substrate alone) for 6 h is relatively small[2]. |
Cell Assay | Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2 : 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through microcentrifuge filter, separateed on HPLC, and analyzed for the fluorogenic AMC moiety[2]. |
References |
Density | 1.4±0.1 g/cm3 |
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Boiling Point | 1200.1±65.0 °C at 760 mmHg |
Molecular Formula | C30H37N5O13 |
Molecular Weight | 675.641 |
Flash Point | 679.6±34.3 °C |
Exact Mass | 675.238770 |
PSA | 287.61000 |
LogP | 1.07 |
Vapour Pressure | 0.0±0.3 mmHg at 25°C |
Index of Refraction | 1.594 |
Storage condition | −20°C |
Water Solubility | DMSO: 10 mM |
Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
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RIDADR | NONH for all modes of transport |
WGK Germany | 3 |