Ac-Asp-Glu-Val-Asp-AMC ammonium salt

Modify Date: 2024-01-02 13:26:41

Ac-Asp-Glu-Val-Asp-AMC ammonium salt structure	Common Name	Ac-Asp-Glu-Val-Asp-AMC ammonium salt
	CAS Number	169332-61-0	Molecular Weight	675.641
	Density	1.4±0.1 g/cm3	Boiling Point	1200.1±65.0 °C at 760 mmHg
	Molecular Formula	C₃₀H₃₇N₅O₁₃	Melting Point	N/A
	MSDS	USA	Flash Point	679.6±34.3 °C

Use of Ac-Asp-Glu-Val-Asp-AMC ammonium salt

Ac-DEVD-AMC is the Caspase-3 substrate. Sequence: N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Methylcoumarin.

Name	N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin
Synonym	More Synonyms

Description	Ac-DEVD-AMC is the Caspase-3 substrate. Sequence: N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Methylcoumarin.
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Peptides Research Areas >> Cancer
In Vitro	Ac-DEVD-AMC is the caspase-3 substrate and could be used to monitor intracellular caspase-3 activity[1].The AMC moiety is highest at 2 h (area =5.6±1.8), the AMC peak area for Ac-DEVD-AMC incubated without lung specimen (substrate alone) for 6 h is relatively small[2].
Cell Assay	Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2 : 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through microcentrifuge filter, separateed on HPLC, and analyzed for the fluorogenic AMC moiety[2].
References	[1]. Ahmed R Alsuwaidi, et al. Bioenergetics of murine lungs infected with respiratory syncytial virus. Virol J. 2013; 10: 22. [2]. Ahmed R Alsuwaidi, et al. Lung tissue bioenergetics and caspase activity in rodents. BMC Res Notes. 2013; 6: 12.

Density	1.4±0.1 g/cm3
Boiling Point	1200.1±65.0 °C at 760 mmHg
Molecular Formula	C₃₀H₃₇N₅O₁₃
Molecular Weight	675.641
Flash Point	679.6±34.3 °C
Exact Mass	675.238770
PSA	287.61000
LogP	1.07
Vapour Pressure	0.0±0.3 mmHg at 25°C
Index of Refraction	1.594
Storage condition	−20°C
Water Solubility	DMSO: 10 mM

Ac-Asp-Glu-Val-Asp-AMC ammonium salt MSDS(Chinese)

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
RIDADR	NONH for all modes of transport
WGK Germany	3

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Ac-DEVD-AMC

MFCD00671411

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