Shanghai Nianxing Industrial Co., Ltd
China
Product Name: BCECF-AM
Price:
Purity: 98.0%
Stocking Period: 10 Day
Contact: Yang

Shanghai Jizhi Biochemical Technology Co., Ltd
China
Product Name: BCECF-AM
Price: ￥2008.0/1mg
Purity: 98.0%
Stocking Period: Inquiry
Contact: Liu jia

DC Chemicals Limited
China
Product Name: BCECF-AM
Price: $Inquiry/100mg ￥Inquiry/250mg ￥Inquiry/1g
Purity: 98.0%
Stocking Period: 1 Day
Contact: Tony Cao

Dayang Chem (Hangzhou) Co., Ltd.
China
Product Name: Bcecf-am
Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
Purity: 98.0%
Stocking Period: Inquiry
Contact: Ms Wang

117464-70-7

117464-70-7 structure

117464-70-7 structure

Name: BCECF-AM
Chemical Name: 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymet
CAS Number: 117464-70-7
Molecular Formula: C₄₀H₃₆O₁₉
Molecular Weight: 820.70
Catalog: Dye Reagents
Create Date: 2018-10-23 16:46:00
Modify Date: 2024-01-02 14:29:30
BCECF-AM is a cell membrane permeable compound, widely used as a fluorescent indicator for intracellular pH.

Name	2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymet
Synonyms	Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoic acid, 3',6'-bis[(acetyloxy)methoxy]-5-[[(acetyloxy)methoxy]carbonyl]-3-oxo-, bis[(acetyloxy)methyl] ester MFCD00036969 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester Acetoxymethyl 3',6'-bis(acetoxymethoxy)-2',7'-bis[3-(acetoxymethoxy)-3-oxopropyl]-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-5-carboxylate 2′,7′-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester Mixed isomers acetyloxymethyl 3',6'-bis(acetyloxymethoxy)-2',7'-bis[3-(acetyloxymethoxy)-3-oxopropyl]-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate BCECF-acetoxymethyl BCECF-AM

Description	BCECF-AM is a cell membrane permeable compound, widely used as a fluorescent indicator for intracellular pH.
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others
In Vitro	BCECF-AM is used to measure changes in basal pHi and NHE activity induced by increasing concentrations of ET-1 (0.1-10 nM) in pulmonary arterial smooth muscle cell (PASMC)[1].
Cell Assay	PASMCs are placed in a laminar flow cell chamber perfused with HBSS with pH adjusted to 7.4. pHi is measured in cells incubated with the membrane permeant (acetoxymethyl ester) form of the pH-sensitive fluorescent dye BCECF-AM for 60 min at 37°C under an atmosphere of 20% O2-5% CO2. Cells are then washed with HBSS for 15 min at 37°C to remove extracellular dye and allow complete de-esterification of cytosolic dye. Ratiometric measurement of BCECF fluorescence is performed on a workstation consisting of a Nikon TSE 100 Ellipse inverted microscope with epi-fluorescence attachments. The light beam from a xenon arc lamp is filtered by interference filters at 490 and 440 nm, and focused onto the PASMCS under examination via a 20× fluorescence objective. Light emitted from the cell at 530 nm is returned through the objective and detected by an imaging camera. An electronic shutter is used to minimize photobleaching of dye. Protocols are executed and data collected on-line with InCyte software. pHi is estimated from in situcalibration after each experiment. Cells are perfused with a solution containing (in mM): 105 KCl, 1 MgCl2, 1.5 CaCl2, 10 glucose, 20 HEPES-Tris and 0.01 nigericin to allow pHi to equilibrate to external pH. A two point calibration is created from fluorescence measured as pHi is adjusted with KOH from 6.5 to 7.5. Intracellular H+ ion concentration ([H+]i) is determined from pHi using the formula: pHi = −log ([H+]i).
References	[1]. Clark Undem, et al. Endothelin-1 Augments Na+/H+ Exchange Activity in Murine Pulmonary Arterial Smooth Muscle Cells via Rho Kinase. PLoS One. 2012; 7(9): e46303.

Density	1.5±0.1 g/cm3
Boiling Point	939.2±65.0 °C at 760 mmHg
Molecular Formula	C₄₀H₃₆O₁₉
Molecular Weight	820.70
Flash Point	368.9±34.3 °C
PSA	264.39000
LogP	0.90
Vapour Pressure	0.0±0.3 mmHg at 25°C
Index of Refraction	1.602
Storage condition	-20°C

Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xi: Irritant;
Risk Phrases	R36/37/38
Safety Phrases	S22-S26-S36
RIDADR	NONH for all modes of transport