305834-79-1
305834-79-1 structure
- Name: SC79
- Chemical Name: 2-Amino-6-chloro-alpha-cyano-3-(ethoxycarbonyl)-4H-1-benzopyran-4-acetic acid ethyl ester
- CAS Number: 305834-79-1
- Molecular Formula: C17H17ClN2O5
- Molecular Weight: 364.780
- Catalog: Signaling Pathways PI3K/Akt/mTOR Akt
- Create Date: 2018-12-25 04:10:31
- Modify Date: 2024-01-02 18:54:54
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SC79 is a selective and cell-permeable Akt activator which activates Akt phosphorylation and inhibits Akt membrane translocation.
| Name | 2-Amino-6-chloro-alpha-cyano-3-(ethoxycarbonyl)-4H-1-benzopyran-4-acetic acid ethyl ester |
|---|---|
| Synonyms |
Ethyl 2-amino-6-chloro-4-(1-cyano-2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate
4H-1-Benzopyran-4-acetic acid, 2-amino-6-chloro-α-cyano-3-(ethoxycarbonyl)-, ethyl ester SC-79 SC79 |
| Description | SC79 is a selective and cell-permeable Akt activator which activates Akt phosphorylation and inhibits Akt membrane translocation. |
|---|---|
| Related Catalog | |
| Target |
Akt |
| In Vitro | SC79 reduces neuronal excitotoxicity and prevents stroke-induced neuronal death. SC79 suppresses PHAKTM-GFP plasma membrane translocation, and enhances phosphorylation of all three Akt isoforms in HEK293, HeLa, HL60, NB4, and HsSulton (B cells) cells[1]. SC79 restores proliferation of BRAT1 knockdown cells, and reduces the production of superoxide in mitochondria of MitoSox positive cells[2]. |
| In Vivo | SC79 (0.04 mg/g, i.p.) inhibits the cytosolic activation of Akt, and recapitulates the primary cellular function of Akt signaling, resulting in augmented neuronal survival, in the permanent focal cerebral ischemia mouse model[1]. |
| Cell Assay | HsSultan or NB4 cells (2.5×105) are plated in a 24-well plate in 500 μL of phenol red-free RPMI medium supplemented with 10% FBS. After incubation for 24 hours, each compound (8 µg/mL) is added and cultured for overnight (16-20 h). Fifty μLs of MTT solution (5 mg/mL in PBS) are added to each well. Following 2 hrs incubation, the purple formazan crystals are dissolved by directly adding in 500 μL of isopropanol with 0.1mol/LHCl to each well. After clearing the cell debris by centrifugation, the absorbance is measured at a wavelength of 570 nm. |
| Animal Admin | The permanent focal cerebral ischemia is induced by middle cerebral artery occlusion (MCAO) essentially. Briefly, mice (C57 Black/6) weighing 17-25 g are anesthetized with 4% isoflurane/66% N2O/30% O2 and maintained with 1.5% isoflurane. Permanent focal ischemia is achieved as follows: a 2-mm hole is drilled at a site superior and lateral to the left foramen ovale to expose the left middle cerebral artery. The proximal portion of the left middle cerebral artery (MCA) is permanently occluded over a 1-mm segment distal to the origin of the lenticulostriate branches through the use of a bipolar coagulator. SC79 is injected intraperitoneally (0.04 mg/g mouse body weight) 5 min before permanent MCAO. In another experiment, extra SC79 is injected (0.04 mg/g mouse body weight, once per hour for 6 hours). |
| References |
| Density | 1.3±0.1 g/cm3 |
|---|---|
| Boiling Point | 524.8±50.0 °C at 760 mmHg |
| Molecular Formula | C17H17ClN2O5 |
| Molecular Weight | 364.780 |
| Flash Point | 271.2±30.1 °C |
| Exact Mass | 364.082611 |
| LogP | 3.23 |
| Vapour Pressure | 0.0±1.4 mmHg at 25°C |
| Index of Refraction | 1.564 |
| Storage condition | -20℃ |
| RIDADR | NONH for all modes of transport |
|---|