145337-55-9
145337-55-9 structure
- Name: Ro 31-9790
- Chemical Name: Ro 31-9790
- CAS Number: 145337-55-9
- Molecular Formula: C15H29N3O4
- Molecular Weight: 315.409
- Catalog: Research Areas Inflammation/Immunology
- Create Date: 2018-05-31 19:02:06
- Modify Date: 2024-01-13 17:52:08
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Ro 31-9790 is a synthetic metalloproteinase (MMP) inhibitor.
| Name | Ro 31-9790 |
|---|---|
| Synonyms |
(2R)-N1-[(2S)-3,3-Dimethyl-1-(methylamino)-1-oxo-2-butanyl]-N4-hydroxy-2-isobutylsuccinamide
Butanediamide, N1-[(1S)-2,2-dimethyl-1-[(methylamino)carbonyl]propyl]-N4-hydroxy-2-(2-methylpropyl)-, (2R)- |
| Description | Ro 31-9790 is a synthetic metalloproteinase (MMP) inhibitor. |
|---|---|
| Related Catalog | |
| Target |
MMP[1] |
| In Vitro | Ro 31-9790 inhibits L-selectin shedding from mouse and human lymphocytes, Jurkat T cells, and human monocytes with an IC50 of 0.3-0.4 μM on these cell types. The IC50 values obtained for Ro 31-9790 are 4.82 ± 0.75 μM, 1.16 ± 0.27 μM, 0.70 ± 0.06 μM, 4.47± 1.27 μM and 0.38 ± 0.05 μM for mouse lymphocyte L-selectin shedding, Jurkat L-selectin shedding, human lymphocyte L-selectin shedding, human monocyte L-selectin shedding and human monocyte TNF-α shedding[1]. |
| Cell Assay | For mouse and human lymphocytes and Jurkat T cells, 100% inhibition is set as the percentage of cells positive for L-selectin in an untreated sample (after flow cytometric analysis) or the concentration of soluble L-selectin in the supernatant of an untreated cell sample (after ELISA); 0% inhibition is set at the appropriate value for a PMA-treated sample. For results from human monocytes, a different calculation is needed to allow direct comparison of L-selectin and TNF-α shedding. Therefore, in this case 100% inhibition is set as the percentage of cells positive for L-selectin in the presence of PMA+50 μM Ro 31-9790 or the percentage of cells positive for cell surface TNF-α in the presence of LPS+50 μM Ro 31-9790 (doses of Ro 31-9790 which gave maximal inhibition); 0% inhibition is set as the percentage of cells positive for L-selectin or TNF-α in the presence of PMA or LPS, respectively. Intermediate percent inhibition is calculated. The IC50 value for inhibitor in each system is defined as the concentration of inhibitor which gave 50% inhibition, where 100% inhibition is set as the percentage of cells positive for L-selectin or cell surface TNF-α in the presence of Phorbol myristate acetate (PMA)+50 μM Ro 31-9790 or Lipopolysaccharide (LPS)+50 μM Ro 31-9790, respectively (doses of Ro 31-9790 which gave maximal inhibition) and 0% inhibition is set as the percentage of cells positive for L-selectin or TNF-α in the presence of PMA or LPS alone, respectively[1]. |
| References |
| Density | 1.1±0.1 g/cm3 |
|---|---|
| Molecular Formula | C15H29N3O4 |
| Molecular Weight | 315.409 |
| Exact Mass | 315.215820 |
| LogP | 0.29 |
| Index of Refraction | 1.484 |
| Storage condition | 2-8℃ |