878674-84-1
878674-84-1 structure
- Name: BODIPY 505/515-8-C3-COOH
- Chemical Name: 4-(4,4-Difluoro-1,3,5,7-tetraMethyl-4-bora-3a,4a-diaza-s-indacene-8-yl)-butyric Acid
- CAS Number: 878674-84-1
- Molecular Formula: C17H22BF2N2O2
- Molecular Weight: 335.1765864
- Catalog: Research Areas Others
- Create Date: 2018-11-12 18:27:25
- Modify Date: 2024-01-10 14:29:29
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BODIPY 505/515-8-C3-COOH is a green fluorescing derivative, as a fluorescent dye for imaging lipid droplets in nannochloropsis. BODIPY 505/515-8-C3-COOH can be used for the research of flow cytometric high-throughput screening and cell sorting[1].
| Name | 4-(4,4-Difluoro-1,3,5,7-tetraMethyl-4-bora-3a,4a-diaza-s-indacene-8-yl)-butyric Acid |
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| Description | BODIPY 505/515-8-C3-COOH is a green fluorescing derivative, as a fluorescent dye for imaging lipid droplets in nannochloropsis. BODIPY 505/515-8-C3-COOH can be used for the research of flow cytometric high-throughput screening and cell sorting[1]. |
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| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Cells: 1. Fresh N. oceanica cultures are diluted to ~4×106 cells/ml with ASW and kept at 22℃ prior to any treatment. Incubate the cells according to your normal protocol. 2. BODIPY 505/515 is dissolved in DMSO at 4 mg/ml and diluted with DMSO to different working stock concentrations. 3. Cell suspensions are supplemented with the appropriate BODIPY 505/515 working stock to a specific DMSO concentration between 2 and 10% (v/v) with final BODIPY concentrations between 0.8 and 4 μg/ml. 4. Pure DMSO was used for control treatments. 1 ml of fresh culture was diluted to ~4×106 cells/ml with ASW and stained with 6% DMSO and 1.2 μg/ml BODIPY for 15 min (non-stressed cultures) or with 10% DMSO and 1.6 μg/ml BODIPY for 36 min (stressed cultures). 5. Upon addition of the dye, samples were vortexed for 5 s and then incubated in the dark for 15 min before flow cytometric analysis, if not indicated otherwise. |
| References |
| Molecular Formula | C17H22BF2N2O2 |
|---|---|
| Molecular Weight | 335.1765864 |