Shanghai Nianxing Industrial Co., Ltd
China
Product Name: QL9?
Price:
Purity: 98.0%
Stocking Period: 10 Day
Contact: Yang

Taiwan Oyi peptide Co. Ltd
China
Product Name: QL9
Price: $Inquiry/1mg $Inquiry/100kg
Purity: 98.0%
Stocking Period: 1 Day
Contact: Vinnie

159646-83-0

159646-83-0 structure

159646-83-0 structure

Name: QL9
Chemical Name: QL9
CAS Number: 159646-83-0
Molecular Formula: C₅₂H₇₄N₁₀O₁₄
Molecular Weight: 1063.21
Catalog: Peptides
Create Date: 2019-02-11 10:31:36
Modify Date: 2024-01-08 17:00:25
QL9 (QLSPFPFDL) is a high-affinity alloantigen for the 2C T cell receptor (TCR).

Name	QL9

Description	QL9 (QLSPFPFDL) is a high-affinity alloantigen for the 2C T cell receptor (TCR).
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Inflammation/Immunology Peptides
Target	TCR[1]
In Vitro	Mouse T cell clone 2C recognizes two different major histocompatibility (MHC) ligands, the self MHC Kb and the allogeneic MHC Ld. Two distinct peptides, SIY (SIYRYYGL) and QL9 (QLSPFPFDL), act as strong and specific agonists when bind to Kb and Ld, respectively. QL9 binding to MHC Ld is influenced by the majority of peptide side chains, distributed across the entire length of the peptide. Findings with both systems, but QL9-Ld in particular, suggest that many single-residue substitutions, introduced into peptides to improve their binding to MHC and thus their vaccine potential, could impair T cell reactivity due to their dual impact on TCR binding. T cell activation assays are performed to measure effects of peptide SIY and QL9 residues on T cell function[2].
Cell Assay	Wild type 2C and high affinity 2C T cell transfectants m67 and m6 are incubated with Kb- or Ld-positive cells and various concentrations of peptide SIY and QL9 alanine variants. T cell activation is measured by assaying for levels of IL-2 release. Briefly, T cell transfectants (7.5×104) are incubated with T2-Kb (7.5×104) or T2-Ld (7.5×104) along with various concentrations of peptide for 20-24 h at 37 °C and 5% CO2. Supernatant is harvested, and levels of IL-2 are measured in an enzyme-linked immunosorbent assay type format. Results are plotted as percentage of maximal IL-2 release=((A450 (sample)-A450(no peptide))/(Max A450(sample)-A450(no peptide)))×100; signal obtained from no peptide is similar to that obtained for the null peptides MCMV or OVA. Binding curves are generated in GraphPad Prism by plotting the percentage of maximal IL-2 release against peptide concentration. The concentrations of peptide yielding 50% maximal IL-2 release (SD50) are calculated using non-linear regression (sigmoidal fitting; GraphPad Prism) of the activation curves[2].
References	[1]. Speir JA, et al. Structural basis of 2C TCR allorecognition of H-2Ld peptide complexes. Immunity. 1998 May;8(5):553-62. [2]. Bowerman NA, et al. Different strategies adopted by K(b) and L(d) to generate T cell specificity directed against their respective bound peptides. J Biol Chem. 2009 Nov 20;284(47):32551-61.

Molecular Formula	C₅₂H₇₄N₁₀O₁₄
Molecular Weight	1063.21
Storage condition	2-8℃