Shanghai Nianxing Industrial Co., Ltd
China
Product Name: BCECF
Price:
Purity: 98.0%
Stocking Period: 10 Day
Contact: Huang

Shanghai Jizhi Biochemical Technology Co., Ltd
China
Product Name: BCECF
Price: ￥838.0/5mg ￥328.0/1mg
Purity: 90.0%
Stocking Period: Inquiry
Contact: Liu jia

85138-49-4

85138-49-4 structure

85138-49-4 structure

Name: BCECF
Chemical Name: 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein
CAS Number: 85138-49-4
Molecular Formula: C₂₇H₂₀O₁₁
Molecular Weight: 520.44100
Catalog: Dye Reagents
Create Date: 2018-07-27 11:48:20
Modify Date: 2024-01-31 22:12:06
BCECF is a duorescent dye with λexcitation of 420~520 nm and λemission of 530 nm.

Name	2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein
Synonyms	2-[2,7-bis(2-carboxyethyl)-3-hydroxy-6-oxoxanthen-9-yl]terephthalic acid BCECF

Description	BCECF is a duorescent dye with λexcitation of 420~520 nm and λemission of 530 nm.
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others
In Vitro	Fully treated cells show hydrogenosomes with an electron-dense deposit which aggregates to a variable extent.The staining is seen in the interior of hydrogenosomes in some instances. It is also observed by microscopy that the K+/H+ ionophor nigericin does not inhibit hydrogenosomal loading with BCECF[1].
Kinase Assay	The cells are palced in a petri dish and BCECF present in trichomonads is fixed for electron microsopy. Controns without BCECF loading, without DAB treatment, and without UV illumination are run in parallel. Trichomonads remaine viable during the procedure, except that without BCECF they are dead and disrupted by 4 h. Then the cell are lysed. After lysis, BCECF is added to the lysate to 20 μM, and the lysate is incubated,followed by 3 washes in lysis buffer to remove free dye and resuspension of the pellet in the original volume[1].
References	[1]. Scott DA, et al. Analysis of the uptake of the fluorescent marker 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF) by hydrogenosomes in Trichomonas vaginalis. Eur J Cell Biol. 1998 Jun;76(2):139-45.

Density	1.67g/cm3
Boiling Point	936.2ºC at 760mmHg
Molecular Formula	C₂₇H₂₀O₁₁
Molecular Weight	520.44100
Flash Point	318.2ºC
Exact Mass	520.10100
PSA	187.89000
LogP	3.39840
Index of Refraction	1.743
Storage condition	-20°C

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	Xn
Risk Phrases	20/21/22-36/37/38
Safety Phrases	22-24/25
RIDADR	NONH for all modes of transport
WGK Germany	3.0