1407523-31-2
1407523-31-2 structure
- Name: Fl-DIBO
- Chemical Name: Fl-DIBO
- CAS Number: 1407523-31-2
- Molecular Formula: C19H12O3
- Molecular Weight: 288.30
- Catalog: Research Areas Others
- Create Date: 2022-09-16 18:55:36
- Modify Date: 2024-01-20 19:39:27
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Fl-DIBO (fluorogenic dibenzocyclooctyne) is a selective and high sensitivity fluorescent probe to azide compounds. Fl-DIBO can react rapidly with azide compounds to form new highly fluorescent products with a maximum emission wavelength of 469 nm and excitation wavelength of 363 nm. Fl-DIBO can be used to label diazo-tagged proteins without detectable background signal interference[1][2].
| Name | Fl-DIBO |
|---|
| Description | Fl-DIBO (fluorogenic dibenzocyclooctyne) is a selective and high sensitivity fluorescent probe to azide compounds. Fl-DIBO can react rapidly with azide compounds to form new highly fluorescent products with a maximum emission wavelength of 469 nm and excitation wavelength of 363 nm. Fl-DIBO can be used to label diazo-tagged proteins without detectable background signal interference[1][2]. |
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| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling diazo-labeled proteins[2][3]: 1. Resuspend the protein with 1×PBS (pH 7.4) and incubate with NHS-activated ester (25 mM in DMSO) overnight at room temperature. 2. Labeled proteins are co-incubated with Fl-DIBO (25-500 μM) at 37 °C for 18 h. 3. Protein SDS-PAGE gel analysis and in-gel fluorescence imaging analysis with λex=365 nm, λem=480 nm. |
| References |
| Molecular Formula | C19H12O3 |
|---|---|
| Molecular Weight | 288.30 |