| In Vitro |
ODN 21158 (0.01, 0.1, 1, 10 μM) shows no cytotoxic in human PBMCs[1]. ODN 21158 (0.01, 0.1, 1, 10 μM; 24 h) inhibits IFN-α secretion in a dose dependent manner when stimulated with CpG-ODN 2216 (3 μM) in Human PBMCs[1]. ODN 21158 (0.01, 0.1, 1, 10 μM; 48 h) stimulated with CpG-ODN 2006 (100 nM) failes to inhibit IL-6 release of PBMCs[1]. ODN 21158 (0.01, 0.1, 1, 10 μM; 48 h) inhibits the release of IL-6 when stimulated with imiquimod (5 μg/ml) in human PBMCs[1]. ODN 21158 (0.1, 1, 10 μM; 24 h) inhibits the release of IL-6 in B-cells when stimulated with imiquimod (5 μg/ml) and CpG-ODN 2006 (100 nM)[1]. Cell Cytotoxicity Assay[1] Cell Line: human PBMCs Concentration: 0.01, 0.1, 1, 10 μM Incubation Time: 48 h Result: Did not result in increased levels of extracellular LDH and not reduced the intracellular ATP-levels of CpG-ODN 2006-stimulated PBMCs.
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