186033-13-6
186033-13-6 structure
- Name: Biotin-16-UTP
- Chemical Name: Biotin-16-UTP
- CAS Number: 186033-13-6
- Molecular Formula: C32H48Li4N7O19P3S
- Molecular Weight: 987.51
- Catalog: Research Areas Others
- Create Date: 2022-11-21 20:45:56
- Modify Date: 2024-08-19 17:44:12
-
Biotin-16-UTP is an active substrate for RNA polymerase. Biotin-16-UTP can replace UTP in the in vitro transcription reaction for RNA labeling[1].
| Name | Biotin-16-UTP |
|---|
| Description | Biotin-16-UTP is an active substrate for RNA polymerase. Biotin-16-UTP can replace UTP in the in vitro transcription reaction for RNA labeling[1]. |
|---|---|
| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). In Vitro RNA Synthesis and Purification: 1. Incubate the cells according to your normal protocol. 2. Add gently One volume of transcription buffer 2x [200 mM KCI, 20 mM Tris-HCI, pH 8.0, 5 mM MgCl2, 4 mM dithiothreitol (DTT), 4 mM each of ATP, GTP and CTP, 200 mM sucrose and 20% glycerol] to nuclei in ice, form mixture. 3. Add 8 μL biotin-16-UTP (from 10 mM tetralithium sal) to the mixture, which is incubated for 30 min at 29°C. 4. Add 6 μL 250 mM CaCl2, 6 μL RNase-free DNase I (10 U/μL) and incubating for 10 min at 29°C to stop reaction. 5. Perform RNA purification of both nuclear run-on and total RNA according to the manufacturer's instructions. 6. Resuspend RNA in 50 uL diethylpyrocarbonate (DEPC)-treated water. 7. Labeled RNA was captured by streptavidin-coated magnetic beads. 8. RNA-binding beads are then used for random hexamer primed reverse transcription. |
| References |
| Molecular Formula | C32H48Li4N7O19P3S |
|---|---|
| Molecular Weight | 987.51 |