| In Vitro |
Taccalonolide E (5 or 10 μM; 18 h) 在 A-10 细胞中导致间期细胞中细胞微管密度增加，形成厚束微管[1]。 Taccalonolide E (1 or 5 μM; 18 h) 导致 A-10 和 HeLa 细胞出现异常多极有丝分裂纺锤体[1]。 Taccalonolide E (5 μM; 6-24 h) 在 G2-M 期阻滞细胞周期[1]。 Taccalonolide E (0.5-5 μM; 18 h) 在间期细胞中启动微核化[1]。 Taccalonolide E (48 h) 抑制癌细胞增殖[1]。 Taccalonolide E (10 μM; 1 h) 导致 MDA-MB-435 细胞微管蛋白聚合[1]。 Taccalonolide E (5 μM; 4-30 h) 启动 Bcl-2 磷酸化，MAPK 激活和凋亡[1]。 Cell Cycle Analysis[1] Cell Line: MDA-MB-435 cells Concentration: 5 μM Incubation Time: 6, 12, 18, or 24 h Result: Caused cells to accumulate in the G2-M phase of the cell cycle. Cell Proliferation Assay[1] Cell Line: SK-OV-3, MDA-MB-435, NCI/ADR, 1 A9, PTX 10, PTX 22 and 1A9/A8 cells Concentration: Incubation Time: 48 h Result: Inhibited the proliferation with IC50s of 0.99 ± 0.08, 0.78 ± 0.17, 21.1 ± 0.46, 0.34 ± 0.04, 1.64 ± 0.25, 4.01 ± 0.20 and 1.42 ± 0.30 μM against SK-OV-3, MDA-MB-435, NCI/ADR, 1 A9, PTX 10, PTX 22 and 1A9/A8 cells, respectively. Western Blot Analysis[1] Cell Line: MDA-MB-435 cells Concentration: 5 μM Incubation Time: 4, 6, 12, 24, or 30 h Result: Activated ERK1/2 and increased PARP cleavage.
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