112926-02-0

112926-02-0 structure

112926-02-0 structure

Name: Indo-1 AM
Chemical Name: acetyloxymethyl 2-[4-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-3-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylate
CAS Number: 112926-02-0
Molecular Formula: C₄₇H₅₁N₃O₂₂
Molecular Weight: 1009.91000
Catalog: Dye Reagents
Create Date: 2018-03-03 08:00:00
Modify Date: 2024-01-24 02:08:11
Indo-1 AM is a fluorescent Ca2+ indicator (λex=340 nm, λem=405/485 nm).

Name	acetyloxymethyl 2-[4-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-3-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylate
Synonyms	MFCD00036955 Indo-1 pentaacetoxymethyl ester INDO 1/AM Indo-1 AM

Description	Indo-1 AM is a fluorescent Ca2+ indicator (λex=340 nm, λem=405/485 nm).
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others
In Vitro	Indo-1 AM is a fluorescent Ca2+ indicator with λex=340 nm and λem=405/485 nm[1]. In rabbit hearts that are exposed to Indo-1 AM, 72% of the dye is in the cytosol, and the remainder is associated with the mitochondria, microsomes and the nucleus[2].
Cell Assay	The cells are loaded with indo-I and fura-2 by exposure to the membrane-permeant forms, Indo-1 AM (indo-l-acetoxymethyl ester) and fura-2-acetoxymethyl ester, respectively. A mixture of 10 μL of 1 mM Indo-1 AM or fura-2/AM in dimethylsulfoxide (DMSO), 2.5 μL of 25% wt/wt Pluronic F-127 in DMSO, and 75 μL fetal calf serum (FCS) or newborn calf serum (NCS) is added to 2 mL Tyrode solution containing the cells and 40 μL FCS or NCS. Loading is achieved by gently shaking the cells for 15 to 30 min[2].
References	[1]. Andrienko T, et al. Real-Time Fluorescence Measurements of ROS and [Ca2+] in Ischemic / Reperfused Rat Hearts: Detectable Increases Occur only after Mitochondrial Pore Opening and Are Attenuated by Ischemic Preconditioning. PLoS One. 2016 Dec 1;11(12):e0167300. [2]. Blatter LA, et al. Intracellular diffusion, binding, and compartmentalization of the fluorescent calcium indicators indo-1 and fura-2. Biophys J. 1990 Dec;58(6):1491-9.

Density	1.374g/cm3
Boiling Point	1009.2ºC at 760mmHg
Molecular Formula	C₄₇H₅₁N₃O₂₂
Molecular Weight	1009.91000
Flash Point	564.1ºC
Exact Mass	1009.30000
PSA	303.73000
LogP	3.14650
Vapour Pressure	3.2E-28mmHg at 25°C
Index of Refraction	1.577

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Safety Phrases	S22-S24/25
RIDADR	NONH for all modes of transport