136-16-3
136-16-3 structure
- Name: Oxythiamine
- Chemical Name: Oxythiamine Chloride
- CAS Number: 136-16-3
- Molecular Formula: C12H16N3O2S
- Molecular Weight: 266.34
- Catalog: Research Areas Cancer
- Create Date: 2018-06-06 23:30:31
- Modify Date: 2024-01-06 21:31:01
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Oxythiamine, an antimetabolite and a vitamin B1 antagonist, is a well-known thiamine antagonist and inhibitor of transketolase.
| Name | Oxythiamine Chloride |
|---|---|
| Synonyms |
Oxythiamine HCl
5-[[5-(2-Hydroxyethyl)-4-Methyl-Thiazol-3-ium-3-yl]Methyl]-2-Methyl-3H-Pyrimidin-4-One Chloride 5-[[5-(2-Hydroxyethyl)-4-Methyl-3-Thiazol-3-iumyl]Methyl]-2-Methyl-3H-Pyrimidin-4-One Chloride 3-[(3,4-Dihydro-2-Methyl-4-Oxo-5-Pyrimidinyl)Methyl]-5-(2-Hydroxyethyl)-4-Methyl-Thiazolium 5-[2-Hydroxyethyl]-3-[4-hydroxy-2-methyl-5-pyrimidinylmethyl]-4-methylthiazolium chloride hydrochloride |
| Description | Oxythiamine, an antimetabolite and a vitamin B1 antagonist, is a well-known thiamine antagonist and inhibitor of transketolase. |
|---|---|
| Related Catalog | |
| Target |
Vitamin B1, Thiamine, Transketolase[1]. |
| In Vitro | Oxythiamine, an antimetabolite and a vitamin B1 antagonist, is a well-known thiamine antagonist and inhibitor of transketolase. Oxythiamine alters protein expression in a dose dependent manner. The level of alpha-enolase is increased by Oxythiamine treatment, while expression of 14-3-3 protein beta/alpha is suppressed by Oxythiamine at a stratified dose. Oxythiamine causes dynamic changes of total protein expression in time dependent fashion. Oxythiamine suppresses expression of cellular phosphor proteins significantly[2]. |
| Cell Assay | Human pancreatic carcinoma cell line MIA PaCa-2 is used throughout the study. Experiments are set up in two groups: dose and time-dependent groups. For the dose-dependent group, the cells are stimulated with 5, 50 and 500 μM Oxythiamine (OT) for 48 hours, respectively. The unstimulated cells are considered as control. For the time-dependent group, the cells are stimulated with 50 μM Oxythiamine in MEM containing natural amino acids or 50% of 15 N algal amino acid mixture for 12 and 48 h. The unstimulated cells are considered as the zero time point. Each treatment is repeated four times with 10 mL/flask. The cell pellets are then collected for further analysis[2]. |
| References |
| Molecular Formula | C12H16N3O2S |
|---|---|
| Molecular Weight | 266.34 |
| PSA | 98.36000 |
| LogP | 2.33510 |
| Storage condition | 2-8℃ |