550-82-3

550-82-3 structure

Name: 3H-Phenoxazin-3-one,7-hydroxy-, 10-oxide
Chemical Name: resazurin
CAS Number: 550-82-3
Molecular Formula: C₁₂H₆NNaO₄
Molecular Weight: 251.17000
Catalog: Research Areas Others
Create Date: 2018-12-28 09:36:05
Modify Date: 2024-01-09 18:53:51
Resazurin (Diazoresorcinol) is a water-soluble, non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal)[1][2].

Name	resazurin
Synonyms	3H-Phenoxazin-3-one,7-hydroxy-,10-oxide azoresorcin 7-Hydroxy-10-oxy-phenoxazin-3-on Diazoresorcinal 7-hydroxy-3h-fenoxazin-3-on-10-oxid 7-hydroxy-3h-phenoxazin-3-on10-oxide PHENOXYAZIN-3-ONE,7-HYDROXY-,10-OXIDE Resazurin tablets 7-HYDROXY-10-OXY-PHENOXAZIN-3-ONE 7-hydroxy-3h-phenoxazin-3-one10-oxide

Description	Resazurin (Diazoresorcinol) is a water-soluble, non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal)[1][2].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Cell viability[1][2]: 1. Defrost the Resazurin solution in a 37°C water bath. 2. Place the cells on a 96-well plate, and washed by PBS (avoid light). 3. Remove the PBS wash, then add 500 μL (1µg/mL) of Resazurin solution. 4. Place the plate, in the incubator for 30 min (incubation time depends on cell type and cell number). 5. Measure Resorufin fluorescence (Ex=530-560 nm, Em=590 nm) using a microplate reader.
References	[1]. Silva FSG, et al. Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay. Curr Protoc Toxicol. 2016 May 4;68:2.24.1-2.24.15. [2]. Rampersad SN. Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays. Sensors (Basel). 2012;12(9):12347-60.

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: NCNSA6 National Academy of Sciences, National Research Council, Chemical-Biological Coordination Center, Review. (Washington, DC) Volume(issue)/page/year: 5,24,1953

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 44,225,1978 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 84815 No. of Facilities: 303 (estimated) No. of Industries: 1 No. of Occupations: 7 No. of Employees: 3485 (estimated) No. of Female Employees: 2576 (estimated)

HS Code	2934999090

HS Code	2934999090
Summary	2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%