3H-Phenoxazin-3-one,7-hydroxy-, 10-oxide

Modify Date: 2024-01-09 18:53:51

3H-Phenoxazin-3-one,7-hydroxy-, 10-oxide Structure
3H-Phenoxazin-3-one,7-hydroxy-, 10-oxide structure
Common Name 3H-Phenoxazin-3-one,7-hydroxy-, 10-oxide
CAS Number 550-82-3 Molecular Weight 251.17000
Density 1.57g/cm3 Boiling Point 477.2ºC at 760mmHg
Molecular Formula C12H6NNaO4 Melting Point N/A
MSDS N/A Flash Point 242.4ºC

 Use of 3H-Phenoxazin-3-one,7-hydroxy-, 10-oxide


Resazurin (Diazoresorcinol) is a water-soluble, non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal)[1][2].

 Names

Name resazurin
Synonym More Synonyms

 3H-Phenoxazin-3-one,7-hydroxy-, 10-oxide Biological Activity

Description Resazurin (Diazoresorcinol) is a water-soluble, non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal)[1][2].
Related Catalog
In Vitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Cell viability[1][2]: 1. Defrost the Resazurin solution in a 37°C water bath. 2. Place the cells on a 96-well plate, and washed by PBS (avoid light). 3. Remove the PBS wash, then add 500 μL (1µg/mL) of Resazurin solution. 4. Place the plate, in the incubator for 30 min (incubation time depends on cell type and cell number). 5. Measure Resorufin fluorescence (Ex=530-560 nm, Em=590 nm) using a microplate reader.
References

[1]. Silva FSG, et al. Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay. Curr Protoc Toxicol. 2016 May 4;68:2.24.1-2.24.15.

[2]. Rampersad SN. Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays. Sensors (Basel). 2012;12(9):12347-60.

 Chemical & Physical Properties

Density 1.57g/cm3
Boiling Point 477.2ºC at 760mmHg
Molecular Formula C12H6NNaO4
Molecular Weight 251.17000
Flash Point 242.4ºC
Exact Mass 251.01900
PSA 78.73000
LogP 2.47010
Index of Refraction 1.74

 Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :
SP7700000
CHEMICAL NAME :
3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide
CAS REGISTRY NUMBER :
550-82-3
BEILSTEIN REFERENCE NO. :
0221382
LAST UPDATED :
199701
DATA ITEMS CITED :
4
MOLECULAR FORMULA :
C12-H7-N-O4
MOLECULAR WEIGHT :
229.20
WISWESSER LINE NOTATION :
T C666 BN FV IOJ BO LQ

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
500 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
NCNSA6 National Academy of Sciences, National Research Council, Chemical-Biological Coordination Center, Review. (Washington, DC) Volume(issue)/page/year: 5,24,1953
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
179 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 44,225,1978 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 84815 No. of Facilities: 303 (estimated) No. of Industries: 1 No. of Occupations: 7 No. of Employees: 3485 (estimated) No. of Female Employees: 2576 (estimated)

 Safety Information

HS Code 2934999090

 Customs

HS Code 2934999090
Summary 2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

 Synonyms

3H-Phenoxazin-3-one,7-hydroxy-,10-oxide
azoresorcin
7-Hydroxy-10-oxy-phenoxazin-3-on
Diazoresorcinal
7-hydroxy-3h-fenoxazin-3-on-10-oxid
7-hydroxy-3h-phenoxazin-3-on10-oxide
PHENOXYAZIN-3-ONE,7-HYDROXY-,10-OXIDE
Resazurin tablets
7-HYDROXY-10-OXY-PHENOXAZIN-3-ONE
7-hydroxy-3h-phenoxazin-3-one10-oxide
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