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1223-07-0

1223-07-0 structure
1223-07-0 structure
  • Name: p-nitrophenyl alpha-l-arabinopyranoside
  • Chemical Name: (2S,3R,4S,5S)-2-(4-nitrophenoxy)oxane-3,4,5-triol
  • CAS Number: 1223-07-0
  • Molecular Formula: C11H13NO7
  • Molecular Weight: 271.22300
  • Catalog: Biochemical Carbohydrate Monosaccharide
  • Create Date: 2018-12-25 17:14:35
  • Modify Date: 2024-01-07 15:34:45
  • p-Nitrophenyl α-L-arabinopyranoside is a biochemical reagent. p-Nitrophenyl α-L-arabinopyranoside can be hydrolyzed by recombinant BgaA (rBgaA, isolated from E. coli BL21 (DE3) strain harboring pEBGA29). p-Nitrophenyl α-L-arabinopyranoside has potential application in enzyme activity detection[1][3].
Name (2S,3R,4S,5S)-2-(4-nitrophenoxy)oxane-3,4,5-triol
Synonyms (2S,3R,4S,5S)-2-(4-nitrophenoxy)tetrahydro-2H-pyran-3,4,5-triol
Description p-Nitrophenyl α-L-arabinopyranoside is a biochemical reagent. p-Nitrophenyl α-L-arabinopyranoside can be hydrolyzed by recombinant BgaA (rBgaA, isolated from E. coli BL21 (DE3) strain harboring pEBGA29). p-Nitrophenyl α-L-arabinopyranoside has potential application in enzyme activity detection[1][3].
Related Catalog
In Vitro p-Nitrophenyl α-L-arabinopyranoside 与 rBgaA 的亲和度 Km 值为 6.06 mM[1]。 p-Nitrophenyl α-L-arabinopyranoside 对 xylosidase–arabinosidase (xarB) 基因具有高活性[2]。 p-Nitrophenyl α-L-arabinopyranoside 在酶活性检测中的应用[3] (1) 200 µL 2 mM p-Nitrophenyl α-L-arabinopyranoside、100 µL 酶、300 µL 50 mM 磷酸盐缓冲液 (pH 7.0)，在 37℃ 下共孵育 0.5、1 和 5 h。 (2) 加入 400 µL 0.5 M 氢氧化钠，终止反应。 (3) 在 405 nm 处测量混合物的吸光度。 注：在人参皂苷存在的情况下，用丁醇萃取终止反应。
References

[1]. Kosugi A, et al. Characterization of two noncellulosomal subunits, ArfA and BgaA, from Clostridium cellulovorans that cooperate with the cellulosome in plant cell wall degradation. J Bacteriol. 2002 Dec;184(24):6859-65.  

[2]. Mai V, et al. Cloning, sequencing, and characterization of the bifunctional xylosidase-arabinosidase from the anaerobic thermophile thermoanaerobacter ethanolicus. Gene. 2000 Apr 18;247(1-2):137-43.  

[3]. Shin H Y, et al. Purification and Characterization ofα-l-Arabinopyranosidase andα-l-Arabinofuranosidase from Bifidobacterium breve K-110, a Human Intestinal Anaerobic Bacterium Metabolizing Ginsenoside Rb2 and Rc[J]. Applied and environmental microbiology, 2003, 69(12): 7116-7123.

Density 1.597g/cm3
Boiling Point 523.2ºC at 760mmHg
Melting Point 205ºC
Molecular Formula C11H13NO7
Molecular Weight 271.22300
Flash Point 270.2ºC
Exact Mass 271.06900
PSA 124.97000
Vapour Pressure 8.94E-12mmHg at 25°C
Index of Refraction 1.654
Storage condition −20°C
Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes Xi
RIDADR NONH for all modes of transport
WGK Germany 3
Precursor  2

DownStream  0


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