Shanghai Nianxing Industrial Co., Ltd
China
Product Name: Dp44mT
Price:
Purity: 98.0%
Stocking Period: 10 Day
Contact: Huang

ShangHai DC Chemicals Co.,Ltd
China
Product Name: Dp44mT
Price: ￥Inquiry/1g
Purity: 98.9%
Stocking Period: 1 Day
Contact: Tony Lai

DC Chemicals Limited
China
Product Name: Dp44mT
Price: $200.0/100mg $450.0/250mg $1000.0/1g
Purity: 98.0%
Stocking Period: 3 Day
Contact: Tony Cao

152095-12-0

152095-12-0 structure

152095-12-0 structure

Name: Dp44mT
Chemical Name: 3-(dipyridin-2-ylmethylideneamino)-1,1-dimethylthiourea
CAS Number: 152095-12-0
Molecular Formula: C₁₄H₁₅N₅S
Molecular Weight: 285.367
Catalog: Research Areas Cancer
Create Date: 2017-06-16 07:20:45
Modify Date: 2024-01-01 22:59:43
Dp44mT is an iron chelator with selective anticancer activity.

Name	3-(dipyridin-2-ylmethylideneamino)-1,1-dimethylthiourea
Synonyms	2-(Di-2-pyridinylmethylene)-N,N-dimethylhydrazinecarbothioamide 2-(dipyridin-2-ylmethylidene)-N,N-dimethylhydrazinecarbothioamide Hydrazinecarbothioamide,2-(di-2-pyridinylmethylene)-N,N-dimethyl Iron Chelator,Dp44mT Hydrazinecarbothioamide, 2-(di-2-pyridinylmethylene)-N,N-dimethyl- 2,N-dimethylsemicarbazone Dp44mT

Description	Dp44mT is an iron chelator with selective anticancer activity.
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Cancer
Target	Target: Iron chelator[1]
In Vitro	Dp44mT is cytotoxic to breast cancer cells, at least in part, due to selective inhibition of top2α. Dp44mT alone induced selective cell killing in the breast cancer cell line MDA-MB-231 when compared with healthy mammary epithelial cells (MCF-12A). It induces G1 cell cycle arrest and reduces cancer cell clonogenic growth at nanomolar concentrations. Dp44mT, but not the iron chelator desferal, induces DNA double-strand breaks quantified as S139 phosphorylated histone foci (γ-H2AX) and Comet tails induced in MDA-MB-231 cells. Doxorubicin-induced cytotoxicity and DNA damage are both enhanced significantly in the presence of low concentrations of Dp44mT. The chelator caused selective poisoning of DNA topoisomerase IIα (top2α) as measured by an in vitro DNA cleavage assay and cellular topoisomerase-DNA complex formation[1]. Dp44mT targets lysosome integrity through copper binding. Copper binding is essential for the potent antitumor activity of Dp44mT, as coincubation with nontoxic copper chelators markedly attenuated its cytotoxicity[2].
Kinase Assay	For DNA topoisomerase IIα assays, the 161-bp fragment from pBluescript SK(-) phagemid DNA or single stranded oligonucleotides are 5'-end labeled with [32P]ATP and T4 polynucleotide kinase. Labeling mixtures are subsequently centrifuged through Mini Quick Spin DNA columns (for pSK fragments) or Oligo columns (for oligonucleotides) to remove the unincorporated label. Annealing to the complementary strand of the oligonucleotides is done by heating the reaction mixture to 95°C and overnight cooling to room temperature in 10 mM Tris-HCl (pH 7.8), 100 mM NaCl, and 1 mM EDTA. DNA substrates (10 pmol/reaction) are incubated with 500 ng of top2a or top2h in the presence or absence of Dp44mT for the indicated times at 25°C in 10 μL of reaction buffer. Reactions are stopped by adding SDS (final concentration 0.5%). Samples are separated on 16% (for pSK DNA) or 20% (for the oligonucleotides) denaturing polyacrylamide gels (7 M urea). Imaging and quantitation are done using a PhosphorImager[1].
Cell Assay	Cell proliferation is measured using a sulforhodamine B dye–based assay. MDA-MB-231(breast cancer) and MCF-12A (healthy mammary epithelial) cells are incubated with increasing concentrations of Dp44mT (0.01, 0.1, 1, 10, 100 μM). Results are expressed relative to control[1].
References	[1]. Rao VA, et al. The iron chelator Dp44mT causes DNA damage and selective inhibition of topoisomerase IIalpha in breast cancer cells. Cancer Res. 2009 Feb 1;69(3):948-57. [2]. Lovejoy DB, et al. Antitumor activity of metal-chelating compound Dp44mT is mediated by formation of a redox-active copper complex that accumulates in lysosomes. Cancer Res. 2011 Sep 1;71(17):5871-80.

Density	1.2±0.1 g/cm3
Boiling Point	438.4±43.0 °C at 760 mmHg
Molecular Formula	C₁₄H₁₅N₅S
Molecular Weight	285.367
Flash Point	218.9±28.2 °C
Exact Mass	285.104828
PSA	92.54000
LogP	1.21
Vapour Pressure	0.0±1.1 mmHg at 25°C
Index of Refraction	1.635
Storage condition	-20℃

Symbol	GHS06
Signal Word	Danger
Hazard Statements	H301
Precautionary Statements	P301 + P310
RIDADR	UN 2811 6.1 / PGIII