- Shanghai Nianxing Industrial Co., Ltd
- China
- Product Name: PHE-THR-ASP-ASN-TYR-THR-ARG-LEU-ARG-LYS-GLN-MET-ALA-VAL-LYS-LYS-TYR-LEU-ASN-SER-ILE-LEU-ASN-NH2
- Price:
- Purity: 98.0%
- Stocking Period: 10 Day
- Contact: Yang
- GL Biochem (Shanghai) Ltd.
- China
- Product Name: VIP (6-28) (human, bovine, porcine, rat)
- Price: $Inquiry/1g
- Purity: 98.0%
- Stocking Period: Inquiry
- Contact: Jackie Yang
- Taiwan Oyi peptide Co. Ltd
- China
- Product Name: VIP (6-28) (human, mouse, rat) trifluoroacetate salt
- Price: $Inquiry/1mg $Inquiry/100kg
- Purity: 98.0%
- Stocking Period: 1 Day
- Contact: Vinnie
69698-54-0
69698-54-0 structure
- Name: VIP (6-28) (human, mouse, rat) trifluoroacetate salt
- Chemical Name: 6-28-Vasoactiveintestinal Octacosapeptide
- CAS Number: 69698-54-0
- Molecular Formula: C126H207N37O34S
- Molecular Weight: 2816.28000
- Catalog: Peptides
- Create Date: 2018-10-08 17:25:20
- Modify Date: 2024-01-02 09:01:25
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VIP(6-28)(human, rat, porcine, bovine) is an effective antagonist of the actions of exogenous vasoactive intestinal peptide (VIP) on cAMP.
| Name | 6-28-Vasoactiveintestinal Octacosapeptide |
|---|---|
| Synonyms |
Vasoactive Intestinal Peptide Fragment 6-28 human,porcine,rat
PHE-THR-ASP-ASN-TYR-THR-ARG-LEU-ARG-LYS-GLN-MET-ALA-VAL-LYS-LYS-TYR-LEU-ASN-SER-ILE-LEU-ASN-NH2 vip 6-28 VIP (6-28) (human, bovine, porcine, rat) VIP(6-28)(human, rat, porcine, bovine) |
| Description | VIP(6-28)(human, rat, porcine, bovine) is an effective antagonist of the actions of exogenous vasoactive intestinal peptide (VIP) on cAMP. |
|---|---|
| Related Catalog | |
| Target |
VIP[1] |
| In Vitro | VIP(6-28) is an effective VIP antagonist in the superior cervical ganglion (SCG) , and results obtained using this analog indicate that endogenous VIP can participate in a positive feedback loop in injured sympathetic neurons in which it enhances its own expression. VIP(6-28), when added to short-term cultures of adult SCG at a concentration of 10, 30, or 100 μM, reduces the increase in cAMP levels produced by stimulation with 10 μM VIP by 52, 64, or 81%, respectively. At any of these concentrations tested, VIP(6-28) by itself does not alter cAMP levels. In contrast to its ability to reduce the VIP-stimulated elevation in cAMP levels by 64%, the addition of 30 μM VIP(6-28) to culture medium does not significantly alter cAMP levels measured after stimulation of adult ganglia with either isoproterenol or forskolin (10 μM each). Similar results on the ability of VIP(6-28) to block VIP-stimulated increases in cAMP levels are obtained in neuron-enriched and in non-neuronal cell-enriched dissociated cultures[1]. |
| Kinase Assay | Adult rats are killed by decapitation. The SCGs are removed, desheathed, placed in organ culture, and maintained for 24 or 48 hr in F-12 defined medium equilibrated with 95% O2 and 5% CO2. Some ganglia are preincubated for 30 min in medium containing the VIP receptor antagonist VIP(6-28), and then transferred for 24 hr to medium containing both VIP(6-28) and an agonist. In experiments in which cAMP is to be measured, ganglia are removed from animals and preincubated for 30 min in F-12 medium containing 500 μM IBMX to prevent the metabolism of cAMP. Ganglia are then incubated for an additional 30 min in F-12 medium with IBMX and the compound to be studied. When the action of VIP(6-28) is examined, it is added to the medium during the last 5 min of the preincubation and throughout the incubation. Ascorbic acid (0.2 mg/mL) is added to cultures containing isoproterenol to retard oxidation of the catecholamine. No significant differences in peptide levels are detected between ganglia maintained in F-12 alone and those cultured in medium containing ascorbic acid[1]. |
| References |
| Molecular Formula | C126H207N37O34S |
|---|---|
| Molecular Weight | 2816.28000 |
| Exact Mass | 2814.53000 |
| PSA | 1247.28000 |
| LogP | 5.53550 |
| Storage condition | -20°C |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| RIDADR | NONH for all modes of transport |