- Shanghai Nianxing Industrial Co., Ltd
- China
- Product Name: CHLOROBIMANE
- Price:
- Purity: 98.0%
- Stocking Period: 10 Day
- Contact: Huang
76421-73-3
76421-73-3 structure
- Name: Monochlorobimane
- Chemical Name: monochlorobimane
- CAS Number: 76421-73-3
- Molecular Formula: C10H11ClN2O2
- Molecular Weight: 226.66000
- Catalog: Dye Reagents
- Create Date: 2018-12-27 20:01:08
- Modify Date: 2024-01-06 18:55:41
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Monochlorobimane is a fluorescent dye (λex=380 nm, λem=470 nm).
| Name | monochlorobimane |
|---|---|
| Synonyms |
7-(chloromethyl)-1,2,6-trimethylpyrazolo[1,2-a]pyrazole-3,5-dione
Chlorobimane Monochlorobimane |
| Description | Monochlorobimane is a fluorescent dye (λex=380 nm, λem=470 nm). |
|---|---|
| Related Catalog | |
| In Vitro | A common technique to measure Glutathione (GSH) in cultured cells is to add Monochlorobimane to the culture medium where it readily enters cells to form a fluorescent GSH-monochlorobimane adduct that can be measured fluorometrically. It is clear that the Monochlorobimane approach gives comparable results to the HPLC technique. Although most laboratories use 100 mM Monochlorobimane in their assay our results show that the Monochlorobimane concentration can be lowered to 25 mM and still accurately measure homogenate GSH content[1]. |
| Kinase Assay | Monochlorobimane (mCB) is added to the second half liver tissue homogenate to a final concentration of 100 mM along with glutathione S-transferase (1 U/mL) obtained from equine liver; the homogenate is then allowed to incubate at room temperature for 30 min. The Glutathione (GSH)-Monochlorobimane adduct is measured in a microtiter reader[1]. |
| References |
| Density | 1.4g/cm3 |
|---|---|
| Boiling Point | 314.9ºC at 760 mmHg |
| Melting Point | 135-136ºC(lit.) |
| Molecular Formula | C10H11ClN2O2 |
| Molecular Weight | 226.66000 |
| Flash Point | 144.2ºC |
| Exact Mass | 226.05100 |
| PSA | 42.96000 |
| LogP | 0.86100 |
| Index of Refraction | 1.61 |
| Symbol |
GHS07 |
|---|---|
| Signal Word | Warning |
| Hazard Statements | H315-H319-H335 |
| Precautionary Statements | P261-P305 + P351 + P338 |
| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Gloves |
| Hazard Codes | Xi: Irritant; |
| Risk Phrases | R36/37/38 |
| Safety Phrases | 26-36 |
| RIDADR | NONH for all modes of transport |
| HS Code | 2933990090 |
| Precursor 3 | |
|---|---|
| DownStream 0 | |
| HS Code | 2933990090 |
|---|---|
| Summary | 2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0% |