- GL Biochem (Shanghai) Ltd.
- China
- Product Name: Ac-DEVD-AFC
- Price: $Inquiry/1g
- Purity: 98.0%
- Stocking Period: Inquiry
- Contact: Jackie Yang
- Zhejiang Hangyu API Co., Ltd
- China
- Product Name: Ac-Asp-Glu-Val-Asp-AFC
- Price: $Inquiry/1kg
- Purity: 99.0%
- Stocking Period: 1 Day
- Contact: Vinnie
201608-14-2
201608-14-2 structure
- Name: Ac-Asp-Glu-Val-Asp-AFC
- Chemical Name: N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin
- CAS Number: 201608-14-2
- Molecular Formula: C30H34F3N5O13
- Molecular Weight: 729.612
- Catalog: Biochemical Peptide
- Create Date: 2019-01-01 16:28:22
- Modify Date: 2024-01-07 16:26:41
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Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm).
| Name | N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin |
|---|---|
| Synonyms |
N-Acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-[2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl]-L-α-asparagine
L-α-Asparagine, N-acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]- Ac-DEVD-AFC MFCD01310970 |
| Description | Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm). |
|---|---|
| Related Catalog | |
| In Vitro | After incubation with Ac-DEVD-AFC for 1 hour, significant increase of caspase-3 activity is observed at 4 hour compare with control. There are no significant increases of caspase-3 activity in Photofrin and LPLI group. The cleavage of Ac-DEVD-AFC in response to caspase-3 activation is remarkably inhibited by shRNA-BimL transfection[1]. |
| Cell Assay | For the detection of caspase-3 activity, PBS washes cell pellets (derive from either the medium or the adherent cells) which are suspended in extract buffer [25 mM HEPES (pH7.4), 0.1% TritonX-l00, 10% glycerol, 5 mM DTT, 1mM phenylmethylsulfonyl fluoride, 10 mg/mL pepstatin, and 10 mg/mL Leupeptin] and vortexed vigorously. 20μl of extract (corresponding to 10% of the sample) are incubated with the caspase-3 fluorogenic substrates Ac-DEVD-AFC at 100 μM final concentration at room temperature, and caspase-3 activity is measured continuously by monitoring the release of fluorigenic AFC at 37°C[1]. |
| References |
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Boiling Point | 1152.7±65.0 °C at 760 mmHg |
| Molecular Formula | C30H34F3N5O13 |
| Molecular Weight | 729.612 |
| Flash Point | 650.9±34.3 °C |
| Exact Mass | 729.210510 |
| PSA | 287.61000 |
| LogP | 1.58 |
| Vapour Pressure | 0.0±0.3 mmHg at 25°C |
| Index of Refraction | 1.575 |
| Storage condition | −20°C |
| Water Solubility | H2O: 1 mg/mL |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |