Ac-Asp-Glu-Val-Asp-AFC

Modify Date: 2024-01-07 16:26:41

Ac-Asp-Glu-Val-Asp-AFC structure	Common Name	Ac-Asp-Glu-Val-Asp-AFC
	CAS Number	201608-14-2	Molecular Weight	729.612
	Density	1.5±0.1 g/cm3	Boiling Point	1152.7±65.0 °C at 760 mmHg
	Molecular Formula	C₃₀H₃₄F₃N₅O₁₃	Melting Point	N/A
	MSDS	Chinese USA	Flash Point	650.9±34.3 °C

Use of Ac-Asp-Glu-Val-Asp-AFC

Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm).

Name	N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin
Synonym	More Synonyms

Description	Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm).
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Cancer Dye Reagents Peptides
In Vitro	After incubation with Ac-DEVD-AFC for 1 hour, significant increase of caspase-3 activity is observed at 4 hour compare with control. There are no significant increases of caspase-3 activity in Photofrin and LPLI group. The cleavage of Ac-DEVD-AFC in response to caspase-3 activation is remarkably inhibited by shRNA-BimL transfection[1].
Cell Assay	For the detection of caspase-3 activity, PBS washes cell pellets (derive from either the medium or the adherent cells) which are suspended in extract buffer [25 mM HEPES (pH7.4), 0.1% TritonX-l00, 10% glycerol, 5 mM DTT, 1mM phenylmethylsulfonyl fluoride, 10 mg/mL pepstatin, and 10 mg/mL Leupeptin] and vortexed vigorously. 20μl of extract (corresponding to 10% of the sample) are incubated with the caspase-3 fluorogenic substrates Ac-DEVD-AFC at 100 μM final concentration at room temperature, and caspase-3 activity is measured continuously by monitoring the release of fluorigenic AFC at 37°C[1].
References	[1]. Wang X, et al. Involvement of Bim in Photofrin-mediated photodynamically induced apoptosis. Cell Physiol Biochem. 2015;35(4):1527-36.

Density	1.5±0.1 g/cm3
Boiling Point	1152.7±65.0 °C at 760 mmHg
Molecular Formula	C₃₀H₃₄F₃N₅O₁₃
Molecular Weight	729.612
Flash Point	650.9±34.3 °C
Exact Mass	729.210510
PSA	287.61000
LogP	1.58
Vapour Pressure	0.0±0.3 mmHg at 25°C
Index of Refraction	1.575
Storage condition	−20°C
Water Solubility	H2O: 1 mg/mL

Ac-Asp-Glu-Val-Asp-AFC MSDS(Chinese)

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
RIDADR	NONH for all modes of transport
WGK Germany	3

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Ac-DEVD-AFC

MFCD01310970