2610-10-8

2610-10-8 structure
2610-10-8 structure

Name direct red 80
Synonyms Duaazol Red B
MFCD00054389
picrosiriusred
Direct Fast Red F3B
C.I. direct red 80
Fast Red BA
SIRIUS RED F3B
Solophenyl red 3BL
Direct Red F3B
SIRIUS RED F3BA
SIRIUS RED
EINECS 220-027-3
Description Direct Red 80 (Sirius Red) is a polyazo dye used principally in staining methods for collagen and amyloid. Direct Red 80 does not release benzidine upon degradation and is safer than many traditional direct dyes[1].
Related Catalog
In Vitro Direct Red 80 (Sirius Red) has two important uses in histopathology: staining collagen from solution in saturated aqueous picric acid and staining amyloid from an alkaline solution. Stained structures are red[1]. Confocal laser microscopy (CLM) is also used to evaluate collagen production and distribution after Direct Red 80 staining. Direct Red 80 is not specific for the different types of collagen. Samples are washed in dH2O, pretreats for 40 min with a solution of 0.2% phosphomolybdic acid (PMA) and incubates for 30 min in a solution consisting of 0.1% Direct red 80 diluted in saturated aqueous picric acid. Finally each sample is washed in 0.01 M HCl for 2 min. PMA is used to make colourless cytoplasm and HCl is used to remove non-specific staining[1].
References

[1]. Dapson RW, et al. Certification procedures for sirius red F3B (CI 35780, Direct red 80). Biotech Histochem. 2011;86(3):133-139.

Density 1.85 g/cm3
Molecular Formula C45H26N10Na6O21S6
Molecular Weight 1373.07000
Exact Mass 1371.90000
PSA 573.95000
LogP 14.76520
Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes Xn
Safety Phrases 22-24/25
RIDADR NONH for all modes of transport
WGK Germany 3