205391-02-2
205391-02-2 structure
- Name: 4,5-Diaminofluorescein diacetate
- Chemical Name: 4,5-Diaminofluorescein diacetate
- CAS Number: 205391-02-2
- Molecular Formula: C24H18N2O7
- Molecular Weight: 446.409
- Catalog: Dye Reagents
- Create Date: 2016-09-29 22:40:09
- Modify Date: 2024-01-05 14:32:50
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DAF 2DA is most widely probe for NO measurement.
| Name | 4,5-Diaminofluorescein diacetate |
|---|---|
| Synonyms |
5,6-Diaminofluorescein diacetate
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)-5,6-diamino- (6'-acetyloxy-5,6-diamino-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate 5,6-Diamino-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetate DAF 2DA |
| Description | DAF 2DA is most widely probe for NO measurement. |
|---|---|
| Related Catalog | |
| In Vitro | Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF 2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF 2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein). |
| References |
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Boiling Point | 719.0±60.0 °C at 760 mmHg |
| Molecular Formula | C24H18N2O7 |
| Molecular Weight | 446.409 |
| Flash Point | 320.0±29.2 °C |
| Exact Mass | 446.111389 |
| PSA | 140.17000 |
| LogP | 1.20 |
| Vapour Pressure | 0.0±2.3 mmHg at 25°C |
| Index of Refraction | 1.721 |
| Storage condition | 2-8℃ |