205391-02-2

205391-02-2 structure

Name	4,5-Diaminofluorescein diacetate
Synonyms	5,6-DIAMINOFLUORESCEIN DIACETATE Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)-5,6-diamino 4,5-DIAMINOFLUORESCEIN DIACETATE 2-(3,6-DIACETYLOXY-9H-XANTHEN-9-YL)-4,5-DIAMINO-BENZOIC ACID DAF 2DA EZSolution DAF-2 diacetate DAF-2 DA 5,6-Diaminofluorescein diacetate (6'-acetyloxy-5,6-diamino-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate 5,6-Diaminofluorescein diacetat 5,6-diamino-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate DAF-2 DA (DMSO solution), fluorescent nitric oxide probe DAF-2 diacetate(solution in DMSO) DAF-2 DA, Fluorescent nitric oxide probe 6-Diaminofluorescein diacetat DAF-2 DA - - Calbiochem DAF-2 DIACETATE 4,5-DIAMINOFLUORORESCEIN DIACETATE 5,6-Diamino-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetate DAF-2 DA, CELL PERMEABLE

Description	DAF 2DA is most widely probe for NO measurement.
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Others Dye Reagents
In Vitro	Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF 2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF 2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein).
References	[1]. Wany A, et al. Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence. Methods Mol Biol. 2016;1424:39-47.

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Risk Phrases	R36/38
Safety Phrases	26
RIDADR	NONH for all modes of transport