129541-41-9

129541-41-9 structure

Name: 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt
Chemical Name: 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt
CAS Number: 129541-41-9
Molecular Formula: C₁₄H₁₂BrClNNaO₇
Molecular Weight: 444.59
Catalog: Biochemical Carbohydrate Monosaccharide
Create Date: 2018-11-23 21:50:12
Modify Date: 2026-07-11 18:32:21
X-Gluc sodium is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system)[1].

Name	5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt
Synonyms	5-Bromo-4-chloro-3-indolyl β- D -glucuronide sodium salt 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronidesodiumsal X-Gluc (sodium) X-Glucuro sodium salt (5-Bromo-4-chloro-3-indolyl-beta-D-glucuro 5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt trihydrate X-GLU NA X-GLCA SODIUM SALT 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronic acid sodium salt X-BETA-D-GLUCURONIC ACID, SODIUM SALT 5-Bromo-4-chloro-3-indolyl Beta-D-glucuronide cyclohexylammonium salt BC-Indicator,X-GlcA,X-glucuronide D-Glucuronide Sodium Salt [for Biochemical ] X-gluc/X-GlcA BCI-GLUCURO, SODIUM SALT BCIG SODIUM SALT X-Gluc Sodium Salt X-GLUCURONIDE SODIUM SALT Cyclohexanamine - 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid (1:1) X-GlcA,X-glucuronide CHA salt 5-BROMO-4-CHLORO-3-INDOLYLB-D-GLUCURONID E 5-Bromo-4-chloro-3-indolyl β-D-Glucopyranosiduronic Acid Cyclohexylammonium Salt X-GLCA 5-Bromo-4-chloro-3-indolyl beta-D-Glucopyranosiduronic Acid Sodium Salt 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronicacid sodium salt 5-BROMO-4-CHLORO-3-INDOLYL-B-D-GLUCURO-N IDE CYCLOHEXYLA.S. X-GLUCURO SODIUM SALT X-Gluc CHA Salt 5-Bromo-4-chloro-3-indolyl-β-D-Glucuronic acid sodium salt trihydrate 5-bromo-4-chloro-4-indoly-β-D-glucuronide 5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid cyclohexylammonium salt X-GLCU 5-BroMo-4-chloro-3-indolyl beta-D-Glucuronide SodiuM Salt X-GlcA Na salt, X-Gluc Na salt Cyclohexanaminium 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronate X-glucuronide CHA salt 5-Bromo-4-chloro-3-indolylb-D-glucuronidesodiumsalttrihydrate X-Beta-d-glcua na 3h2o 5-Bromo-4-chloro-3-indolyl-b-D-glucuronide sodium salt 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt trihydrate 5-Bromo-4-chlo 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt //BP X-GlcA CHA Salt 5-Bromo-4-chloro-3-indolyl-D-glucuronic acid - s 5-BROMO-4-CHLORO-3-INDOXYL-BETA-D-GLUCURONIC ACID, SODIUM SALT 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide cyclohexylammonium salt MFCD00135782 X-GlcA CHA salt, X-Gluc CHA salt 5-BROMO-4-CHLORO-3-INDOLYL-BETA-D -GLUCURONIDE SODIUM SALT 5-Bromo-4-chloro-3-indolyl beta-D-Glucuronide Sodium Salt [for Biochemical ] X-GLUCURONIDE 5-BROMO-4-CHLORO-3-INDOLYL BETA-D-GLUCURONIDE SODIUM SALT 5-Bromo-4-chloro-3-indolyl β-D-Glucopyranosiduronic Acid Sodium Salt X-GLUC, NA X-BETA-D-GLCA NA 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt trihydrate BCI-BETA-D-GLUCURONIDE, SODIUM SALT 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt hydrate 5-BROMO-4-CHLORO-3-INDOLYL-BETA-D -GLUCURONIDE MOLECULBIOLOGY SODIUM SALT BC-Indicator, X-GlcA, X-glucuronide (2S,3S,4S,5R,6S)-6-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-3,4,5-trihydroxyoxane-2-cboxylic acid X-Glu,CHA Salt 5-BROMO-4-CHLORO-3-INDOLYL-BETA-D-GLUCURONIDE NA SALT 5-BroMo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodiuM salt anhydrous X-BETA-D-GLUCURONIDE SODIUM SALT BC-INDICATOR 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide Sodium Anhydrous (X-Gluc Sodium) extrapure 5-Bromo-4-chloro-3-indolyl β-D-Glucuronide Sodium Salt 5-Bromo-4-chloro-3-indolyl beta-D-Glucuronide Sodium Salt [for Biochemical ]> 5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt 5-BROMO-4-CHLORO-3-INDOLYL B-D-GLUCURONI DE SODIUM X-GlcA CHA salt/X-Gluc CHA salt /BC-Indicator X-GlcA X-glucuronide 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronic acid cyclohexylammonium salt X-GlcA Sodium Salt 5-BROMO-4-CHLORO-3-INDOLYL-BETA-D-GLUCURONIC ACID 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt 5-BROMO-4-CHLORO-3-INDOLYL-β-D-GLUCORONIDE SODIUM SALT 5-Bromo-4-chloro-3-indolyl &beta 5-BROMO-4-CHLORO-3-INDOLYL-B-D-GLUCURONIDE NA SALT X-GLCA, NA 5-Bromo-4-chloro-3-indolyl beta-D-Glucopyranosiduronic Acid Sodium Salt X-Gluc Sodium Salt X-GlcA Sodium Salt 5-Bromo-4-chloro-3-indolyl β-D-Glucuronide Sodium Salt [for Biochemical ] X-GLUC SODIUM SALT β-D-Glucopyranosiduronic acid, 5-bromo-4-chloro-1H-indol-3-yl, compd. with cyclohexanamine (1:1)

Description	X-Gluc sodium is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system)[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Dissolve 20 mg X-Gluc sodium in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix. 2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization. 3. Allow the plates to air dry and be used to inoculate the organisms. 4. Incubate the plates at 35°C and observe 16-24 h after inoculation.
References	[1]. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404.

Boiling Point	732.2ºC at 760mmHg
Melting Point	228-230°C
Molecular Formula	C₁₄H₁₂BrClNNaO₇
Molecular Weight	444.59
Flash Point	396.6ºC
Exact Mass	442.93834
PSA	135.07000
Storage condition	−20°C

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	Xi
Risk Phrases	R36/37/38
Safety Phrases	S24/25
RIDADR	NONH for all modes of transport
WGK Germany	3
HS Code	29339990

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