IR813
Modify Date: 2024-01-07 17:52:57
IR813 structure
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Common Name | IR813 | ||
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| CAS Number | 134127-48-3 | Molecular Weight | 755.40600 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C47H47ClN2O3S | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
Use of IR813IR 813 tosylate is a near-infrared (NIR) fluorescent dye (λex=815 nm, λem=840 nm) and can be used for visualizing regional lymph nodes in mice[1]. |
| Name | 1h-benz[e]indolium, 2-[2-[2-chloro-3-[(1,3-dihydro-1,1,3-trimethyl-2h-benz[e]indol-2-ylidene)ethylidene]-1-cyclohexen-1-yl]ethenyl]-1,1,3-trimethyl-, salt with 4-methylbenzenesulfonic acid (1:1) |
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| Synonym | More Synonyms |
| Description | IR 813 tosylate is a near-infrared (NIR) fluorescent dye (λex=815 nm, λem=840 nm) and can be used for visualizing regional lymph nodes in mice[1]. |
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| Related Catalog | |
| In Vitro | IR 813 (17.3 μM, 2 h) tosylate induces a 31.4% hemolysis in red blood cells[1]. IR 813 (5.9 μM, 24 h) tosylate induces 50% cell death in MRC-5 cells[1]. |
| In Vivo | IR 813 tosylate displays a maximum fluorescence intensity at 4 h postinjection together with a rapid extravasation, when being used for visualizing regional lymph nodes in mice[1]. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1]. 1. Animal: Ten-week-old female Balb/cOlaAnN mice are kept in a 12 h light/dark cycle to reduce tissue autofluorescence in the NIR region, and had access to food and water ad libitum. 2. Dose: A single dose 5.1 nmol of IR 813 dye (20 μL of 0.173 mg/mL, dissolved in a PEG-400/ethanol/water=3:2:5, v/v/v solution) is subcutaneous injected in the right anterior paw of mice. 3. Imaging: Using the Fluobeam700 NIR imaging system to perform in vivo optical imaging (739 nm excitation light (3.5 mW), 750 nm long-pass emission cutoff filter).Fluorescence intensity in the axillary lymph node (ALN) is recorded for 1 week (5 min, 1 h, 4 h, 24 h, 7 days). 4. Data analysis: Semiquantitative data is obtained from the fluorescence images using ImageJ 1.44 software. |
| Molecular Formula | C47H47ClN2O3S |
|---|---|
| Molecular Weight | 755.40600 |
| Exact Mass | 754.30000 |
| PSA | 71.83000 |
| LogP | 12.47360 |
|
~81%
IR813 CAS#:134127-48-3 |
| Literature: EASTMAN KODAK COMPANY; WIDZINSKI, Thomas, J.; PAWLIK, Thomas, D.; OLM, Myra, Toffolon; BOSE, Judith, A. Patent: WO2013/55640 A2, 2013 ; Location in patent: Page/Page column 25 ; |
![4-methylbenzenesulfonate,1,2,3,3-tetramethylbenzo[e]indol-3-ium structure](https://www.chemsrc.com/caspic/490/141914-99-0.png)
![N-[(3-(Anilinomethylene)-2-chloro-1-cyclohexen-1-yl)methylene]aniline (hydrochloride) structure](https://www.chemsrc.com/caspic/300/63857-00-1.png)
| Precursor 2 | |
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| DownStream 0 | |
| EC 2117 |
| 2-(2-(2-Chloro-3-(2-(1,3-dihydro-1,1,3-trimethyl-2H-benzo[e]indole-2-ylidene)ethylidene)-1-cyclohexen-1-yl)ethenyl]-1] |
| ADS 830A |
| 2-[2-[2-Chloro-3-[2-(1,3-dihydro-1,1,3-trimethyl-2H-benz[e]indol-2-ylidene)ethylidene]-1-cyclohexen-1-yl]ethenyl]-1,1,3-trimethyl-1H-benz[e]indolium salt with 4-methylbenzenesulfonate (1:1) |
| 2,6-DIMETHYLANILINE,[RING-14C(U)] HYDROCHLORIDE |
| IR DYE 813ABS |
| 2-(2-(2-CHLORO-3-[2-(1,1,3-TRIMETHYL-2,3-DIHYDRO-1H-BENZO[E]INDOL-2-YLIDEN)ETHYLIDENE]-1-CYCLOHEXENYL)-1-ETHENYL)-1,1,3-TRIMETHYL-1H-BENZO[E]INDOLIUM 4-METHYL-1-BENZENESULFONATE |
| SDB 7047 |
| 2-[2-[2-CHLORO-3-[2-(1,3-DIHYDRO-1,1,3-TRIMETHYL-2H-BENZO[E]-INDOLE-2-YLIDENE)-ETHYLIDENE]-1-CYCLOHEXEN-1-YL]-ETHENYL]-1,1,3-TRIMETHYL-1H-BENZO[E]INDOLIUM 4-METHYLBENZENESULFONATE |
| IR813 |