NHS-5(6)Carboxyrhodamine

Modify Date: 2025-08-25 18:31:48

NHS-5(6)Carboxyrhodamine Structure
NHS-5(6)Carboxyrhodamine structure
Common Name NHS-5(6)Carboxyrhodamine
CAS Number 150408-83-6 Molecular Weight 527.525
Density 1.5±0.1 g/cm3 Boiling Point 759.4±70.0 °C at 760 mmHg
Molecular Formula C29H25N3O7 Melting Point N/A
MSDS Chinese USA Flash Point 413.1±35.7 °C

 Use of NHS-5(6)Carboxyrhodamine


NHS-5(6)Carboxyrhodamine is a dye used for fluorescence labeling applications, where accurate dye/protein ratios can be obtained under native conditions[1].

 Names

Name 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester
Synonym More Synonyms

 NHS-5(6)Carboxyrhodamine Biological Activity

Description NHS-5(6)Carboxyrhodamine is a dye used for fluorescence labeling applications, where accurate dye/protein ratios can be obtained under native conditions[1].
Related Catalog
In Vitro A general characteristic for all of the NHS-5(6)Carboxyrhodamine/ConA samples is that the 556 nm peak is significantly larger relative to the 520 nm peak[1]. Dye/protein ratios for NHS-5(6)Carboxyrhodamine under native conditions are close to the ratios obtained under denaturing conditions[1].
References

[1]. D L Meadows, et al. Determining the extent of labeling for tetramethylrhodamine protein conjugates. J Immunol Methods. 1991 Oct 25;143(2):263-72.

 Chemical & Physical Properties

Density 1.5±0.1 g/cm3
Boiling Point 759.4±70.0 °C at 760 mmHg
Molecular Formula C29H25N3O7
Molecular Weight 527.525
Flash Point 413.1±35.7 °C
Exact Mass 527.169250
PSA 105.69000
LogP 2.36
Vapour Pressure 0.0±2.6 mmHg at 25°C
Index of Refraction 1.710
Storage condition -20°C

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes F: Flammable;
RIDADR NONH for all modes of transport
WGK Germany 3

 Articles5

More Articles
Topological stability and self-association of a completely hydrophobic model transmembrane helix in lipid bilayers.

Biochemistry 41 , 3073-3080, (2002)

Investigation of interactions between hydrophobic model peptides and lipid bilayers is perhaps the only way to elucidate the principles of the folding and stability of membrane proteins (White, S. H.,...

Two-dimensional fluorescence intensity distribution analysis: theory and applications.

Biophys. J. 78 , 1703-1713, (2000)

A method of sample analysis is presented which is based on fitting a joint distribution of photon count numbers. In experiments, fluorescence from a microscopic volume containing a fluctuating number ...

E. Koller

Appl. Fluoresc. Technol. 3 , 20, (1991)

 Synonyms

1-({[3',6'-Bis(dimethylamino)-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-5-yl]carbonyl}oxy)-2,5-pyrrolidinedione
(2,5-dioxopyrrolidin-1-yl) 3',6'-bis(dimethylamino)-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate
2,5-Pyrrolidinedione, 1-[[[3',6'-bis(dimethylamino)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]oxy]-
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