Tetrazolium blue

Modify Date: 2024-01-02 17:20:33

Tetrazolium blue Structure
Tetrazolium blue structure
Common Name Tetrazolium blue
CAS Number 1871-22-3 Molecular Weight 727.641
Density N/A Boiling Point N/A
Molecular Formula C40H32Cl2N8O2 Melting Point 255 °C (dec.)(lit.)
MSDS Chinese USA Flash Point N/A
Symbol GHS08
GHS08
Signal Word Danger

 Use of Tetrazolium blue


Blue Tetrazolium (Tetrazolium blue), as a blue dye for microbial research, can be reduced into blue tetrazolium formazan (BTF). Blue Tetrazolium can be used to determine the activity of succinate dehydrogenase (SDH) in yeast strains, which has been reduced as a substrate. Use DMSO to extract BTF from cells, and test absorption spectrum of BTF. The BTF shows a wide wavelength range of 480-600 nm with maximal absorbance seen at 540 nm. Blue Tetrazolium, combines with succinate dehydrogenase (SDH)[1].

 Names

Name tetrazolium blue
Synonym More Synonyms

 Tetrazolium blue Biological Activity

Description Blue Tetrazolium (Tetrazolium blue), as a blue dye for microbial research, can be reduced into blue tetrazolium formazan (BTF). Blue Tetrazolium can be used to determine the activity of succinate dehydrogenase (SDH) in yeast strains, which has been reduced as a substrate. Use DMSO to extract BTF from cells, and test absorption spectrum of BTF. The BTF shows a wide wavelength range of 480-600 nm with maximal absorbance seen at 540 nm. Blue Tetrazolium, combines with succinate dehydrogenase (SDH)[1].
Related Catalog
References

[1]. Kregiel D, et al. Succinate dehydrogenase activity assay in situ with blue tetrazolium salt in crabtree-positive Saccharomyces cerevisiae strain[J]. Food Technology and Biotechnology, 2008, 46(4): 376-380.

 Chemical & Physical Properties

Melting Point 255 °C (dec.)(lit.)
Molecular Formula C40H32Cl2N8O2
Molecular Weight 727.641
Exact Mass 726.202515
PSA 87.64000
LogP 0.42760
Storage condition -20°C
Stability Stable, but may be light sensitive. Incompatible with strong oxidizing agents.

 Safety Information

Symbol GHS08
GHS08
Signal Word Danger
Hazard Statements H350
Precautionary Statements P201-P308 + P313
Personal Protective Equipment Eyeshields;full-face particle respirator type N100 (US);Gloves;respirator cartridge type N100 (US);type P1 (EN143) respirator filter;type P3 (EN 143) respirator cartridges
Hazard Codes T:Toxic;
Risk Phrases R45
Safety Phrases S53-S45
RIDADR NONH for all modes of transport
WGK Germany 3
RTECS XF8050000
HS Code 29339990

 Synthetic Route

~%

Tetrazolium blue Structure

Tetrazolium blue

CAS#:1871-22-3

Literature: Justus Liebigs Annalen der Chemie, , vol. 581, p. 16,22 Cancer Research, , vol. 10, p. 113,114

 Precursor & DownStream

Precursor  1

DownStream  0

 Customs

HS Code 29339990

 Articles112

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 Synonyms

2,2'-(3,3'-dimethoxybiphenyl-4,4'-diyl)bis(3,5-diphenyl-2H-tetrazol-3-ium) dichloride
3,3'-Dianisolebis[4,4'-(3,5-diphenyl)tetrazolium Chloride]
2H-Tetrazolium, 3,3'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[2,5-diphenyl-, chloride (1:2)
Ditetrazolium chloride
3,3'-(3,3'-Dimethoxy[1,1'-biphenyl]-4,4'-diyl)-bis(2,5-diphenyl-2H-tetrazolium) dichloride
BT
EINECS 217-488-8
Tetrazolium Blue Chloride
BT [VAN]
BT(dye)
Blue tetrazolium BT
blue tetrazolium chloride
Dimethoxy neotetrazolium
Xen Tari
3,3'-(3,3'-Dimethoxybiphenyl-4,4'-diyl)bis(2,5-diphenyl-2H-tetrazol-3-ium) dichloride
Blue Tetrazolium
3,3'-(3,3'-dimethoxy-4,4'-biphenylene)bis(2,5-diphenyl-2H-tetrazolium chloride)
Bactospeine
Tetr
3,3'-Dianisole-4,4'-bis(3,5-diphenyltetrazolium chloride)
Turex
3,3'-(3,3'-Dimethoxy-4,4'-biphenyldiyl)bis(2,5-diphenyl-2H-tetrazol-3-ium) dichloride
3,3'-(3,3'-Dimethoxy-[1,1'-biphenyl]-4,4'-diyl)bis(2,5-diphenyl-2H-tetrazol-3-ium) chloride
3,3'-(3,3'-Dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[2,5-diphenyl-2H-tetrazolium] Dichloride
MFCD00040933
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