DiFMUP

Modify Date: 2024-01-22 18:14:06

DiFMUP Structure
DiFMUP structure
Common Name DiFMUP
CAS Number 214491-43-7 Molecular Weight 292.13000
Density N/A Boiling Point N/A
Molecular Formula C10H7F2O6P Melting Point N/A
MSDS N/A Flash Point N/A

 Use of DiFMUP


DiFMUP is a fluorogenic substrate, and has been widely used for the continuous detection of phosphatase activities. DiFMUP is hydrolysis by a phosphatase results in the release of Xuorescent DIFMU, which can be easily followed in continuous mode by a Xuorescence reader[1][2].

 Names

Name 6,8-difluoro-4-methyl-umbelliferyl phosphate
Synonym More Synonyms

 DiFMUP Biological Activity

Description DiFMUP is a fluorogenic substrate, and has been widely used for the continuous detection of phosphatase activities. DiFMUP is hydrolysis by a phosphatase results in the release of Xuorescent DIFMU, which can be easily followed in continuous mode by a Xuorescence reader[1][2].
Related Catalog
In Vitro DIFMUP (100 μM; 0-4 min) is hydrolyzed by protein tyrosine phosphatase 1B (PTP1B) in a time-dependent manner and the initial rate of the reaction velocity is increased with the enzyme concentration (30-600 ng/mL)[2].
References

[1]. Gee KR, et, al. Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and beta-galactosidases. Anal Biochem. 1999 Aug 15;273(1):41-8.

[2]. Welte S, et, al. 6,8-Difluoro-4-methylumbiliferyl phosphate: a fluorogenic substrate for protein tyrosine phosphatases. Anal Biochem. 2005 Mar 1;338(1):32-8.

 Chemical & Physical Properties

Molecular Formula C10H7F2O6P
Molecular Weight 292.13000
Exact Mass 291.99500
PSA 106.78000
LogP 1.85110
Storage condition -20°C

 Safety Information

Safety Phrases 22-24/25
HS Code 2932209090

 Customs

HS Code 2932209090
Summary 2932209090. other lactones. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

 Synonyms

aurora ka-6718
difmup
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