BODIPY TR Cadaverine

Modify Date: 2024-04-05 21:23:34

BODIPY TR Cadaverine Structure
BODIPY TR Cadaverine structure
Common Name BODIPY TR Cadaverine
CAS Number 217190-24-4 Molecular Weight 544.85
Density N/A Boiling Point N/A
Molecular Formula C26H28BClF2N4O2S Melting Point N/A
MSDS N/A Flash Point N/A

 Use of BODIPY TR Cadaverine


BODIPY TR Cadaverine, a cadaverine derivative, is a red fluorescent dye. BODIPY TR Cadaverine can be used in a a highly sensitive and robust fluorescent displacement assay, which binds to native LPS strongly, specifically recognizing lipid A, and is competitively displaced by compounds displaying an affinity for lipid A[1][2].

 Names

Name BODIPY TR Cadaverine

 BODIPY TR Cadaverine Biological Activity

Description BODIPY TR Cadaverine, a cadaverine derivative, is a red fluorescent dye. BODIPY TR Cadaverine can be used in a a highly sensitive and robust fluorescent displacement assay, which binds to native LPS strongly, specifically recognizing lipid A, and is competitively displaced by compounds displaying an affinity for lipid A[1][2].
Related Catalog
In Vitro BODIPY TR Cadaverine assay[1] (to quantify autophagy in cells): Cells were incubated with 125 nM BODIPY TR Cadaverine for 10 minutes, washed four times with PBS and lysed in 10 mM Tris-Cl pH 8, 0.1 % Triton X-100. Fluorescence levels were read in a SPECTRAmax fluorimeter (Molecular Devices) (λex=588 nm, λem=616 nm). Fluorescence levels were normalized for cell number by adding 0.2 mM ethidium bromide and reading DNA fluorescence (λex=530 nm, λem=590 nm)[1].
References

[1]. Carreira RS, et al. Cyclophilin D is required for mitochondrial removal by autophagy in cardiac cells. Autophagy. 2010 May;6(4):462-72.

[2]. Wood SJ, et al. Anti-endotoxin agents. 1. Development of a fluorescent probe displacement method optimized for the rapid identification of lipopolysaccharide-binding agents. Comb Chem High Throughput Screen. 2004 May;7(3):239-49.

 Chemical & Physical Properties

Molecular Formula C26H28BClF2N4O2S
Molecular Weight 544.85