| In Vitro |
Adebrelimab (SHR-1316; 0.06- 1mg/mL; 48 hours) 抑制 SK-BR-3 和 AU565 细胞的增殖、迁移、侵袭[1]。 Adebrelimab（SHR-1316；0.1-1mg/mL；48 小时）下调 PD-L1、p-PI3K、p-AKT 的表达并上调 FOXO1 的表达[1]。SK-BR-3 and AU565 cells 0.1 mg/mL, 1 mg/mL48 hoursPromoted cell apoptosis.SK-BR-3 and AU565 cells 0.1 mg/mL, 1 mg/mL24 hoursDecreased the migration rate of SK-BR -3 and AU565 cells.SK-BR-3 and AU565 cells 0.1 mg/mL, 1 mg/mL48 hoursDownregulated the expression of PD-L1, p-PI3K, p-AKT and upregulated the expression FOXO1. Cell Viability Assay[1] Cell Line: SK-BR-3 and AU565 cells Concentration: 0.06 mg/mL, 0.13 mg/mL, 0.25 mg/mL, 0.5 mg/mL, 1 mg/mL Incubation Time: 48 hours Result: The viability of SK-BR-3 and AU565 cells decreased gradually. Apoptosis Analysis[1] Cell Line: SK-BR-3 and AU565 cells Concentration: 0.1 mg/mL, 1 mg/mL Incubation Time: 48 hours Result: Promoted cell apoptosis. Cell Migration Assay [1] Cell Line: SK-BR-3 and AU565 cells Concentration: 0.1 mg/mL, 1 mg/mL Incubation Time: 48 hours Result: Decreased the migration rate of SK-BR -3 and AU565 cells. Western Blot Analysis[1] Cell Line: SK-BR-3 and AU565 cells Concentration: 0.1 mg/mL, 1 mg/mL Incubation Time: 48 hours Result: Downregulated the expression of PD-L1, p-PI3K, p-AKT and upregulated the expression FOXO1.
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