L-Ascorbic acid 2-phosphate

Modify Date: 2024-01-02 01:17:07

L-Ascorbic acid 2-phosphate structure	Common Name	L-Ascorbic acid 2-phosphate
	CAS Number	23313-12-4	Molecular Weight	256.10400
	Density	2.01 g/cm3	Boiling Point	561.3ºC at 760 mmHg
	Molecular Formula	C₆H₉O₉P	Melting Point	N/A
	MSDS	N/A	Flash Point	293.2ºC

Use of L-Ascorbic acid 2-phosphate

L-ascorbic acid 2-phosphate (2-Phospho-L-ascorbic acid) is a long-acting vitamin C derivative that can stimulate collagen formation and expression[1]. L-ascorbic acid 2-phosphate (2-Phospho-L-ascorbic acid) can be used as a culture medium supplement for the osteogenic differentiation of human adipose stem cells (hASCs). L-ascorbic acid 2-phosphate increases alkaline phosphatase (ALP) activity and expression of runx2A in hASCs during the osteogenic differentiation[2][3].

Name	[2-(1,2-dihydroxyethyl)-3-hydroxy-5-oxo-2H-furan-4-yl] dihydrogen phosphate
Synonym	More Synonyms

Description	L-ascorbic acid 2-phosphate (2-Phospho-L-ascorbic acid) is a long-acting vitamin C derivative that can stimulate collagen formation and expression[1]. L-ascorbic acid 2-phosphate (2-Phospho-L-ascorbic acid) can be used as a culture medium supplement for the osteogenic differentiation of human adipose stem cells (hASCs). L-ascorbic acid 2-phosphate increases alkaline phosphatase (ALP) activity and expression of runx2A in hASCs during the osteogenic differentiation[2][3].
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Metabolic Disease
Target	IC50: alkaline phosphatase (ALP); collagen formation; runx2A[2][3]
In Vitro	L-Ascorbic acid 2-phosphate (0.1-1.5 mM; 2 to 3 weeks with medium exchange every 2 to 3 days) significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc) achieves only weak growth stimulation. A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation with EGF and/or insulin does not have any additional effect [1]. L-Ascorbic acid 2-phosphate (50 µM-250 µM) is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity. The highest proliferation, ALP activity and runx2 expression is achieved with 150 µM AsA2-P and 10 nM dexamethasone (Dex), and 250 µM AsA2-P and 5 nM Dex[3]. Cell Proliferation Assay[1] Cell Line: Human corneal endothelial cells (HCECs) Concentration: 0.1 mM; 0.3 mM; 1.5 mM Incubation Time: 2 to 3 weeks with medium exchange every 2 to 3 days Result: Stimulated HCEC cells growth.
References	[1]. Shima N, et al. Increased proliferation and replicative lifespan of isolated human corneal endothelial cells with L-ascorbic acid 2-phosphate.Invest Ophthalmol Vis Sci. 2011 Nov 7;52(12):8711-7. [2]. Kurata S, et al. Epidermal growth factor inhibits transcription of type I collagen genes and production of type I collagen in cultured human skin fibroblasts in the presence and absence of L-ascorbic acid 2-phosphate, a long-acting vitamin C derivative.J Biol Chem. 1991 May 25;266(15):9997-10003. [3]. Kyllönen L, et al. Effects of different serum conditions on osteogenic differentiation of human adipose stem cells in vitro.Stem Cell Res Ther. 2013 Feb 15;4(1):17.

Density	2.01 g/cm3
Boiling Point	561.3ºC at 760 mmHg
Molecular Formula	C₆H₉O₉P
Molecular Weight	256.10400
Flash Point	293.2ºC
Exact Mass	255.99800
PSA	163.56000
Vapour Pressure	6.19E-15mmHg at 25°C
Index of Refraction	1.629