ABTS
Modify Date: 2024-01-02 16:18:40
ABTS structure
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Common Name | ABTS | ||
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| CAS Number | 30931-67-0 | Molecular Weight | 548.680 | |
| Density | N/A | Boiling Point | 881.2ºC at 760mmHg | |
| Molecular Formula | C18H24N6O6S4 | Melting Point | N/A | |
| MSDS | Chinese USA | Flash Point | 486.7ºC | |
Use of ABTSABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate. |
| Name | Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) |
|---|---|
| Synonym | More Synonyms |
| Description | ABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate. |
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| Related Catalog | |
| In Vitro | A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, as a substrate for HRP conjugate is studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for HRP in terms of sensitivity, ABTS is the most sensitive, stable and the best in visuality by its bluish-green color[1]. ABTS is a typical peroxidase substrate. For purification and characterization peroxidase positive transformants are cultivated in large scale (XL) under conditions that yield active protein in the culture supernatant. After 160 h cultivation an activity of 55,000 U/L in relation to the substrate ABTS is achieved and the supernatant containing the peroxidase is harvested. With ABTS as substrate the peroxidase activity falls significantly when the H2O2 concentration rose above 0.125 mM, indicating that the enzyme is inhibited by H2O2. Maximum reaction rates depending upon substrate tested reache values between 31.2 and 125 µM[2]. |
| Kinase Assay | Enzyme activity is determined photometrically using a temperature controlled multi-mode plate reader or alternatively in a UV/Vis spectrophotometer. Reactions are initiated by addition of the enzyme. Enzyme activity is measured over a period of 10 min at 25°C at the appropriate wavelength for the substrate. One unit (1 U) is defined as the amount of enzyme that converts 1 µmol substrate per minute. Various H2O2 concentrations (0-1250 µM, enzyme concentrations (0.27-54 nM) and substrate concentrations are used to determine the enzyme activity. The activity of rPsaDyP vs ABTS is determined in 100 mM sodium acetate buffer at pH 3.8 and a final H2O2 concentration of 125 µM. Production of the ABTS cation radical is studied at 420 nm (ε420 36,000 L mol-1 cm-1)[2]. |
| References |
| Boiling Point | 881.2ºC at 760mmHg |
|---|---|
| Molecular Formula | C18H24N6O6S4 |
| Molecular Weight | 548.680 |
| Flash Point | 486.7ºC |
| Exact Mass | 548.064026 |
| PSA | 223.04000 |
| LogP | 5.47820 |
| Water Solubility | H2O: 50 mg/mL, very slightly hazy, green |
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Section I.Chemical Product and Company Identification Chemical NameABTS [=2,2'-Azinobis(3-ethylbenzothiazoline- 6-sulfonic Acid Ammonium Salt)] [for Biochemical Research] Portland OR Synonym2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt)
Chemical FormulaC18H24N6O6S4 CAS Number30931-67-0 Section II.Composition and Information on Ingredients CAS Number Percent (%)TLV/PELToxicology Data Chemical Name ABTS [=2,2'-Azinobis(3-ethylbenzothiazoline-Min. 98.0 (N) Not available.Not available. 30931-67-0 6-sulfonic Acid Ammonium Salt)] [for Biochemical Research] Section III. Hazards Identification Irritating to eyes and skin on contact. Inhalation causes irritation of the lungs and respiratory system. Inflammation of the Acute Health Effects eye is characterized by redness, watering, and itching. Skin inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering. Follow safe industrial hygiene practices and always wear proper protective equipment when handling this compound. CARCINOGENIC EFFECTS : Not available. Chronic Health Effects MUTAGENIC EFFECTS : Not available. TERATOGENIC EFFECTS : Not available. DEVELOPMENTAL TOXICITY: Not available. Repeated or prolonged exposure to this compound is not known to aggravate existing medical conditions. Section IV.First Aid Measures Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15 Eye Contact minutes. COLD water may be used. Get medical attention. Skin ContactIn case of contact, immediately flush skin with plenty of water. Remove contaminated clothing and shoes. COLD water may be used.Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention. InhalationIf the victim is not breathing, perform mouth-to-mouth resuscitation. Loosen tight clothing such as a collar, tie, belt or waistband. If breathing is difficult, oxygen can be administered. Seek medical attention if respiration problems do not improve. IngestionINDUCE VOMITING by sticking finger in throat. Lower the head so that the vomit will not reenter the mouth and throat. Loosen tight clothing such as a collar, tie, belt or waistband. If the victim is not breathing, perform mouth-to-mouth resuscitation. Examine the lips and mouth to ascertain whether the tissues are damaged, a possible indication that the toxic material was ingested; the absence of such signs, however, is not conclusive. Section V.Fire and Explosion Data Not available. Auto-Ignition FlammabilityMay be combustible at high temperature. Flammable LimitsNot available. Flash PointsNot available. Combustion ProductsThese products are toxic carbon oxides (CO, CO2), nitrogen oxides (NO, NO2), sulfur oxides (SO2, SO3...). Fire Hazards Not available. Risks of explosion of the product in presence of mechanical impact: Not available. Explosion Hazards Risks of explosion of the product in presence of static discharge: Not available. Fire Fighting Media SMALL FIRE: Use DRY chemical powder. LARGE FIRE: Use water spray, fog or foam. DO NOT use water jet. and Instructions Consult with local fire authorities before attempting large scale fire-fighting operations. Continued on Next Page ABTS [=2,2'-Azinobis(3-ethylbenzothiazoline- 6-sulfonic Acid Ammonium Salt)] [for Biochemical Research] Section VI.Accidental Release Measures Spill CleanupIrritating Material. Use a shovel to put the material into a convenient waste disposal container. Finish cleaning the spill by rinsing any Instructions contaminated surfaces with copious amounts of water. Consult federal, state, and/or local authorities for assistance on disposal. Section VII. Handling and Storage IRRITANT. Keep away from heat. Mechanical exhaust required. When not in use, tightly seal the container and store in a Handling and Storage dry, cool place. Avoid excessive heat and light. Do not breathe dust. Information Always store away from incompatible compounds such as oxidizing agents. Section VIII. Exposure Controls/Personal Protection Engineering ControlsUse process enclosures, local exhaust ventilation, or other engineering controls to keep airborne levels below recommended exposure limits. If user operations generate dust, fume or mist, use ventilation to keep exposure to airborne contaminants below the exposure limit. Personal ProtectionSplash goggles. Lab coat. Boots. Gloves. Suggested protective clothing might not be sufficient; consult a specialist BEFORE handling this product. Be sure to use a MSHA/NIOSH approved respirator or equivalent. Not available. Exposure Limits Section IX. Physical and Chemical Properties Physical state @ 20°CSolid. (Crystalline powder. White to slightlySolubilitySoluble in cold water. pale green.) Not available. Specific Gravity Molecular Weight548.68Partition CoefficientNot available. Boiling PointNot available.Vapor PressureNot applicable. Melting Point300°C (572°F)Vapor DensityNot available. Not available.Not available. Refractive IndexVolatility Not available. Critical TemperatureNot available.Odor ViscosityNot available.TasteNot available. Section X.Stability and Reactivity Data Stability This material is stable if stored under proper conditions. (See Section VII for instructions) Conditions of InstabilityAvoid excessive heat and light. Incompatibilities Reactive with oxidizing agents. Section XI. Toxicological Information RTECS NumberDL7002000 Eye Contact. Ingestion. Inhalation. Routes of Exposure Toxicity DataNot available. CARCINOGENIC EFFECTS : Not available. Chronic Toxic Effects MUTAGENIC EFFECTS : Not available. TERATOGENIC EFFECTS : Not available. DEVELOPMENTAL TOXICITY: Not available. Repeated or prolonged exposure to this compound is not known to aggravate existing medical conditions. Acute Toxic EffectsIrritating to eyes and skin on contact. Inhalation causes irritation of the lungs and respiratory system. Inflammation of the eye is characterized by redness, watering, and itching. Skin inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering. Follow safe industrial hygiene practices and always wear proper protective equipment when handling this compound. Continued on Next Page ABTS [=2,2'-Azinobis(3-ethylbenzothiazoline- 6-sulfonic Acid Ammonium Salt)] [for Biochemical Research] Section XII.Ecological Information Not available. Ecotoxicity Environmental FateNot available. Section XIII. Disposal Considerations Recycle to process, if possible. Consult your local regional authorities. You may be able to dissolve or mix material with a Waste Disposal combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber system. Observe all federal, state and local regulations when disposing of the substance. Section XIV. Transport Information DOT ClassificationNot a DOT controlled material (United States). PIN NumberNot applicable. Proper Shipping NameNot applicable. Packing Group (PG)Not applicable. DOT Pictograms Section XV. Other Regulatory Information and Pictograms TSCA Chemical InventoryThis product is NOT on the EPA Toxic Substances Control Act (TSCA) inventory. The following notices are required by 40 CFR 720.36 (C) for those products not on the inventory list: (EPA) (i) These products are supplied solely for use in research and development by or under the supervision of a technically qualified individual as defined in 40 CFR 720.0 et sec. (ii) The health risks of these products have not been fully determined. Any information that is or becomes available will be supplied on an MSDS sheet. WHMIS ClassificationOn DSL. (Canada) EINECS Number (EEC) 250-396-6 EEC Risk Statements R36/37/38- Irritating to eyes, respiratory system and skin. SECTION 16 - ADDITIONAL INFORMATION N/A |
CHEMICAL IDENTIFICATION
HEALTH HAZARD DATAACUTE TOXICITY DATAMUTATION DATA
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| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Gloves |
|---|---|
| Hazard Codes | Xi: Irritant; |
| Risk Phrases | R36/37/38 |
| Safety Phrases | S26-S36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| RTECS | DL7002000 |
| HS Code | 29342080 |
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| 2,2'-Azinobis(3-ethylbenzothiazolin-6-sulfonsaeure)diammoniumsalz |
| AzBTS-(NH4)2 |
| 6-Benzothiazolesulfonate, 2,2'-[(1Z,2Z)-1,2-hydrazinediylidene]bis[3-ethyl-2,3-dihydro-, (2Z,2'Z)-, ammonium salt (1:2) |
| 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt |
| ABTS (diammonium salt) |
| ABTS SUBSTRATE |
| (2Z,2'Z)-2,2'-(1Z,2Z)-Hydrazine-1,2-diylidenebis(3-ethyl-2,3-dihydro-1,3-benzothiazole-6-sulfonic acid) diammoniate |
| AzBTS-(NH4)2/ABTS diaMMoniuM salt |
| diammonium 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonate) |
| ABTS ammonium salt |
| ABTS [=2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt)] |
| MFCD00010404 |
| 2,2'-azinobis-(3-ethyl-2,3-dihydrobenzothiazole-6-sulphonate) diammonium salt |
| 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt |
| 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt) |
| ABTS-(NH4)2,PACKAGE WITH 50 TABLETS |
| ABTS-(NH4)2 |
| 2,2'-(1,4-PHENYLENE)BIS-1H-BENZOTHIAZOLE |
| EINECS 250-396-6 |
| ABTS(R),DIAMMONIUM SALT |
| Diammonium (2Z,2'Z)-2,2'-[(1Z,2Z)-1,2-hydrazinediylidene]bis(3-ethyl-2,3-dihydro-1,3-benzothiazole-6-sulfonate) |
| ABTS substrate tablets |
| ABTS |