Phenol Red sodium salt structure 
             | 
        Common Name | Phenol Red sodium salt | ||
|---|---|---|---|---|
| CAS Number | 34487-61-1 | Molecular Weight | 375.351 | |
| Density | 0.972 | Boiling Point | 562.8ºC at 760mmHg | |
| Molecular Formula | C19H13NaO5S | Melting Point | 285 °C (dec.)(lit.) | |
| MSDS | Chinese USA | Flash Point | 294.2ºC | |
| Symbol | 
             
            
            GHS07  | 
        Signal Word | Warning | |
            Use of Phenol Red sodium saltPhenol Red sodium salt is a water soluble pH indicator used in the 6.8 (yellow) to 8.2 (red) range.  | 
    
| Name | sodium,4-[3-(4-hydroxyphenyl)-1,1-dioxo-2,1λ6-benzoxathiol-3-yl]phenolate | 
|---|---|
| Synonym | More Synonyms | 
| Description | Phenol Red sodium salt is a water soluble pH indicator used in the 6.8 (yellow) to 8.2 (red) range. | 
|---|---|
| Related Catalog | |
| In Vitro | Phenol Red sodium salt is commonly employed as a cell tissue culture pH marker. Under normal conditions, phenol red added to the growth medium will have a pink-red color. Dying cells or overgrowth of contaminants will cause a change in pH resulting in a change in indicator color. Phenol red bears a structural resemblance to some nonsteroidal estrogens and has significant estrogenic activity at the concentrations (15-45 μM) at which it is found in tissue culture media. Phenol red binds to the estrogen receptor of MCF-7 human breast cancer cells and stimulates the proliferation of estrogen receptor-positive MCF-7 breast cancer cells in a dose-dependent manner but has no effect on the growth of estrogen receptor-negative MDA-MB-231 breast cancer cells[1]. Phenol red is a simple, rapid and inexpensive method for the measurement of hydrogen peroxide produced by cells in culture. The assay is based on the horseradish peroxidase-mediated oxidation of phenol red by hydrogen peroxide which results in the formation of a compound demonstrating increased absorbance at 610 nm[2]. | 
| Cell Assay | Phenol red is dissolved in 0.1% ethanol. To determine the effect of phenol red on cell proliferation, MCF-7 cells grown for 1 week before experiments in phenol red-free MEM supplemented are harvested and seeded into T-25 flasks (ca. 1.5 x 105 cells per flask). The following day, cells from three flasks are harvested and counted with a Coulter Counter. Then the medium is changed to phenol red- and insulin-free MEM, which contains various concentrations of dextran-coated charcoal-treated calf serum, phenol red (0-300 μM), tamoxifen, hydroxytamoxifen, estradiol, or ethanol vehicle (0.1%), and cell number is monitored as a function of time[1]. Phenol Red sodium salt is dissolved in distilled water as a 10 g/L stock solution[2]. | 
| References | 
| Density | 0.972 | 
|---|---|
| Boiling Point | 562.8ºC at 760mmHg | 
| Melting Point | 285 °C (dec.)(lit.) | 
| Molecular Formula | C19H13NaO5S | 
| Molecular Weight | 375.351 | 
| Flash Point | 294.2ºC | 
| Exact Mass | 375.030853 | 
| PSA | 95.04000 | 
| LogP | 4.62760 | 
| Storage condition | Store at RT. | 
| Stability | Stable. Incompatible with strong oxidizing agents. | 
| Water Solubility | soluble | 
| Symbol | 
                                    
                                     
                                    
                                    GHS07  | 
                            
|---|---|
| Signal Word | Warning | 
| Hazard Statements | H315-H319-H335 | 
| Precautionary Statements | P261-P305 + P351 + P338 | 
| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Gloves | 
| Hazard Codes | Xi:Irritant; | 
| Risk Phrases | R36/37/38 | 
| Safety Phrases | S26-S36-S37/39 | 
| RIDADR | NONH for all modes of transport | 
| WGK Germany | 3 | 
| HS Code | 29349990 | 
| Precursor 0 | |
|---|---|
| DownStream 1 | |
| HS Code | 29349990 | 
|---|
| Phenolsulfonephthalein sodium salt | 
| Phenol Red,sodium salt | 
| PR sodium salt | 
| phenolsulfonphthalein sodium salt | 
| phenolsulphophthaleine sodium salt | 
| UNII-50J9732WBG | 
| MFCD00066901 | 
| Phenol, 4,4'-(1,1-dioxido-3H-2,1-benzoxathiol-3-ylidene)bis-, monosodium salt | 
| EINECS 252-057-8 | 
| phenolsulfonephthalein sodium | 
| Sodium 4-(3-(4-hydroxyphenyl)-1,1-dioxido-3H-benzo[c][1,2]oxathiol-3-yl)phenolate | 
| Phenolate, 4,4'-(1,1-dioxido-3H-2,1-benzoxathiol-3-ylidene)bis-, sodium salt (1:1) | 
| phenol red sodium salt | 
| PHENOL RED,ACS | 
| Sodium Phenol Red | 
| sodium hydrogen 4,4'-(3H-2,1-benzoxathiol-3-ylidene)bisphenolate S,S-dioxide | 
| Phenol Red (sodium salt) |