Benzol-1,2,4,5-tetramintetrahydrochlorid

Modify Date: 2024-01-03 08:05:36

Benzol-1,2,4,5-tetramintetrahydrochlorid Structure
Benzol-1,2,4,5-tetramintetrahydrochlorid structure
Common Name Benzol-1,2,4,5-tetramintetrahydrochlorid
CAS Number 4506-66-5 Molecular Weight 284.014
Density N/A Boiling Point 400.9ºC at 760mmHg
Molecular Formula C6H14Cl4N4 Melting Point ≥300ºC(lit.)
MSDS Chinese USA Flash Point 233.6ºC
Symbol GHS07
GHS07
Signal Word Warning

 Use of Benzol-1,2,4,5-tetramintetrahydrochlorid


Y15 is a direct and specific inhibitor of FAK auto-phosphorylation.

 Names

Name benzene-1,2,4,5-tetramine,tetrahydrochloride
Synonym More Synonyms

 Benzol-1,2,4,5-tetramintetrahydrochlorid Biological Activity

Description Y15 is a direct and specific inhibitor of FAK auto-phosphorylation.
Related Catalog
Target

FAK[1]

In Vitro Y15 directly blocks autophosphorylation activity of FAK. Y15 inhibits Y397 phosphorylation of FAK starting at 0.1 μM in Panc-1 cells. At a dose of 100 μM, Y15 has the same or better inhibition as TAE226. Of note, total FAK is downregulated at higher doses of Y15. Y15 also blocks phosphorylation of the FAK downstream substrate, paxillin. Total paxillin is decreased at higher doses similar to FAK. Thus, Y15 inhibits FAK phosphorylation in a dose-dependent manner[1]. MTS assay is completed using a range of Y15 doses on all cell lines (TT, K1, BCPAP, and TPC1, respectively).Y15 inhibited cell viability in a dose-dependent manner across all thyroid cell lines evaluated. IC50 is 2.05, 5.74, 9.99, and 17.54 μM for TT, TPC1, BCPAP, and K1, respectively[2].
In Vivo Nude mice bearing Panc si-ctrl xenografts are treated with TAE226, a dual FAK and IGF-1R tyrosine kinase inhibitor, to provide a reference for the antitumor effect of dual inhibition of FAK and IGF-1R. Without drug treatment, Panc si5-IGF-1R xenografts grew slower than Panc si-ctrl xenografts (Panc si5-IGF-1R/PBS vs. Panc si-ctrl/PBS), suggesting moderate tumor suppression by inhibiting the IGF-1R pathway only. Further inhibition of FAK activity by Y15 treatment suppresses the growth of Panc si5-IGF-1R xenografts more drastically (Panc si5-IGF-1R/PBS vs. Panc si5-IGF-1R/Y15). A similar antitumor effect is seen in Panc si-ctrl xenografts treated with TAE226 (Panc si5-IGF-1R/Y15 vs. Panc si-ctrl/TAE226). Mice demonstrates normal grooming and eating habits throughout the experiment[3].
Kinase Assay 10 μCi of [γ-32P]-ATP in a kinase buffer 20 mM HEPES, pH 7.4, 5 mM MgCl2, 5 mM MnCl2, 0.1 mM Na3VO4 with 0.1 μg of purified FAK protein are incubated in a kinase buffer with 10 μCi of [γ-32P]-ATP. The kinase reaction is performed for 5 minutes at room temperature and stopped by addition of 2× Laemmli buffer. Proteins are separated on a Ready SDS-10% PAGE gel, and the phosphorylated enolase is visualized by autoradiography[1]
Cell Assay The cells are treated with Y15 or TAE226 at different concentrations for 24 hours. The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium compound from Promega Viability kit is added, and the cells are incubated at 37°C for 1-2 hours. The optical density on 96-plate is analyzed with a microplate reader at 490 nm to determine cell viability. In addition, cells are stained with trypan blue after 24 hours of treatment with Y15 and the percent of cells that stained positive are determined with a hemacytometer[1]
Animal Admin Mice[3] Six-week-old, female nude mice are used. 5×106 Panc si5-IGF-1R cells are mixed with matrigel and injected subcutaneously into the flank of athymic nude mice (day 0). Animals are randomly divided into two groups on day 7. One group (n=5) received Y15 (30 mg/kg) and the other received PBS as control treatment (n=5). For Panc si-ctrl xenografts, 5×106 Panc si-ctrl cells are mixed with matrigel and injected subcutaneously into the flank of nude mice. These animals are also randomly divided into two groups on day 7, and one group (n=5) received TAE226 (30 mg/kg), the other group received PBS (n=5) as control treatment. The drugs and PBS are administered through intraperitoneal injection in a total volume of 0.1 mL. Tumor sizes are measured every 3 or 4 d in length (mm)×width (mm) starting from day 10. Tumor volume is calculated as volume (cm3)= ½×length (cm)×width(cm)2.
References

[1]. Hochwald SN, et al. A novel small molecule inhibitor of FAK decreases growth of human pancreatic cancer. Cell Cycle. 2009 Aug;8(15):2435-43.

[2]. O'Brien S, et al. FAK inhibition with small molecule inhibitor Y15 decreases viability, clonogenicity, and cell attachment in thyroid cancer cell lines and synergizes with targeted therapeutics. Oncotarget. 2014 Sep 15;5(17):7945-59.

[3]. Zheng D, et al. A novel strategy to inhibit FAK and IGF-1R decreases growth of pancreatic cancer xenografts. Mol Carcinog. 2010 Feb;49(2):200-9.

 Chemical & Physical Properties

Boiling Point 400.9ºC at 760mmHg
Melting Point ≥300ºC(lit.)
Molecular Formula C6H14Cl4N4
Molecular Weight 284.014
Flash Point 233.6ºC
Exact Mass 281.997253
PSA 104.08000
LogP 5.54820
Index of Refraction 1.827
Storage condition Desiccate at RT
Water Solubility H2O: soluble20mg/mL, clear

 Safety Information

Symbol GHS07
GHS07
Signal Word Warning
Hazard Statements H315-H319-H335
Precautionary Statements P261-P305 + P351 + P338
Personal Protective Equipment dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes Xi
Risk Phrases R36/37/38
Safety Phrases S26
RIDADR NONH for all modes of transport
WGK Germany 3
HS Code 2921590090

 Synthetic Route

~82%

Benzol-1,2,4,5-tetramintetrahydrochlorid Structure

Benzol-1,2,4,5-...

CAS#:4506-66-5

Literature: E I DU PONT DE NEMOURS AND COMPANY; GOLDFINGER, MARC B.; PELLENBARG, TIMOTHY Patent: US2014/66629 A1, 2014 ; Location in patent: Paragraph 0073-0075 ;

 Customs

HS Code 2921590090
Summary 2921590090. other aromatic polyamines and their derivatives; salts thereof. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:30.0%

 Articles7

More Articles
Down-regulation of ALDH1A3, CD44 or MDR1 sensitizes resistant cancer cells to FAK autophosphorylation inhibitor Y15.

J. Cancer Res. Clin. Oncol. 141 , 1613-31, (2015)

Focal adhesion kinase is an important survival signal in cancer. Recently, we demonstrated that the autophosphorylation inhibitor of FAK, Y15, effectively inhibited cancer cell growth. We detected man...

FAK is a critical regulator of neuroblastoma liver metastasis.

Oncotarget 3 , 1576-87, (2013)

Neuroblastomas express increased levels of gastrin-releasing peptide receptor (GRP-R). However, the exact molecular mechanisms involved in GRP-R-mediated cell signaling in neuroblastoma growth and met...

CEACAM6 promotes tumor angiogenesis and vasculogenic mimicry in gastric cancer via FAK signaling.

Biochim. Biophys. Acta 1852(5) , 1020-8, (2015)

CEACAM6 is a member of glycosylphosphatidylinositol-linked immunoglobulin superfamily that is implicated in a variety of human cancers. In our previous study, we reported that CEACAM6 was overexpresse...

 Synonyms

Benzene-1,2,4,5-tetrayltetraamine tetrahydrochloride
1,2,4,5-tetraaminobenzene tetra-hydrochloride
1,2,4,5-Benzenetetramine, tetrahydrochloride
Benzene-1,2,4,5-tetramine tetrahydrochloride
EINECS 224-822-6
MFCD00012970
Benzol-1,2,4,5-tetramintetrahydrochlorid
1,2,4,5-BENZENETETRAMINE TETRAHYDROCHLORIDE
1,2,4,5-Benzenetetramine, hydrochloride (1:4)
Benzene-1,2,4,5-tetraamine tetrahydrochloride
1,2,4,5-Benzenetetraamine tetrahydrochloride
1,2,4,5-tetraaminobenzene tetahydrochloride
Y15