ci 50206

Modify Date: 2025-08-27 18:32:40

ci 50206 Structure
ci 50206 structure
 Common Name ci 50206
CAS Number 4569-86-2 Molecular Weight 378.89800
Density N/A Boiling Point N/A
Molecular Formula C22H23ClN4 Melting Point 285 °C (dec.)(lit.)
MSDS Chinese USA Flash Point N/A

 Use of ci 50206


Methylene Violet 3RAX is a phenazine dye to stain the mitochondria of cells. Methylene Violet 3RAX can change the molecular structure of DNA, undermine the module of DNA, and induce the generation of the reactive singlet oxygen. Methylene Violet 3RAX shows inhibition for human erythrocyte AChE and human plasma BChE with an Kis of 1.58, 0.51 μM, respectively. Methylene Violet 3RAX has the potential for the research of potential photosensitizers for mitochondrial targeting action in PDT (photodynamic therapy)[1][2].

 Names

Name 2-N,2-N-diethyl-10-phenylphenazin-10-ium-2,8-diamine,chloride
Synonym More Synonyms

 ci 50206 Biological Activity

Description Methylene Violet 3RAX is a phenazine dye to stain the mitochondria of cells. Methylene Violet 3RAX can change the molecular structure of DNA, undermine the module of DNA, and induce the generation of the reactive singlet oxygen. Methylene Violet 3RAX shows inhibition for human erythrocyte AChE and human plasma BChE with an Kis of 1.58, 0.51 μM, respectively. Methylene Violet 3RAX has the potential for the research of potential photosensitizers for mitochondrial targeting action in PDT (photodynamic therapy)[1][2].
Related Catalog
Target

Acetylcholinesterase:1.58 μM (Ki)

Butyrylcholinesterase:0.51 μM (Ki)

In Vitro Methylene Violet 3RAX shows antitumor activity by cutting off DNA strands in tumour cell[1].Methylene Violet 3RAX (0-400 µM) significantly enhances cleavage of DNA with over 60% photocleavage activity towards DNA at 300 μM[1].
References

[1]. Si Yao, et al. Methylene violet 3RAX-conjugated porphyrin for photodynamic therapy: synthesis, DNA photocleavage, and cell study. RSC Advances. 2022,12, 21004-21013.

[2]. Onder S, et al. The kinetics of inhibition of human acetylcholinesterase and butyrylcholinesterase by methylene violet 3RAX. Chem Biol Interact. 2019 Dec 1;314:108845.

 Chemical & Physical Properties

Melting Point 285 °C (dec.)(lit.)
Molecular Formula C22H23ClN4
Molecular Weight 378.89800
Exact Mass 378.16100
PSA 46.03000
LogP 1.67830
InChIKey MOVNSGGBTSIUGX-UHFFFAOYSA-N
SMILES CCN(CC)c1ccc2nc3ccc(N)cc3[n+](-c3ccccc3)c2c1.[Cl-]

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Safety Phrases S24/25
RIDADR NONH for all modes of transport
WGK Germany 3
HS Code 2933990090

 Customs

HS Code 2933990090
Summary 2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

 ci 50206Bioassay

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Name: Luminescence-based cell-based primary high throughput screening assay to identify ago...
Source: The Scripps Research Institute Molecular Screening Center
Target: mu-type opioid receptor isoform MOR-1 [Homo sapiens]
External Id: OPRM1-OPRD1_AG_LUMI_1536_1X%ACT PRUN
Name: QFRET-based biochemical primary high throughput screening assay to identify exosite i...
Source: The Scripps Research Institute Molecular Screening Center
Target: disintegrin and metalloproteinase domain-containing protein 17 preproprotein [Homo sapiens]
External Id: ADAM17_INH_QFRET_1536_1X%INH PRUN
Name: Fluorescence-based cell-based primary high throughput screening assay to identify ago...
Source: The Scripps Research Institute Molecular Screening Center
Target: muscarinic acetylcholine receptor M1 [Homo sapiens]
External Id: CHRM1_AG_FLUO8_1536_1X%ACT PRUN
Name: uHTS identification of small molecule activators of the adaptive arm of the Unfolded ...
Source: Burnham Center for Chemical Genomics
Target: N/A
External Id: BCCG-A405-UPR-XBP1-PrimaryAgonist-Assay
Name: Fluorescence polarization to screen for inhibitor that competite the binding of FadD2...
Source: Broad Institute
Target: FATTY-ACID-CoA LIGASE FADD28 (FATTY-ACID-CoA SYNTHETASE)
External Id: 2147-01_Inhibitor_SinglePoint_HTS_Activity
Name: Bursicon-induced LGR2 mediated cAMP production in LGR-2/CRE6x-Luciferase co-transfect...
Source: Broad Institute
Target: N/A
External Id: Bursicon-induced LGR2 mediated cAMP production in LGR-2/CRE6x-Luciferase co-transfected HEK293 cells Inhibition - 7011-01_Antagonist_SinglePoint_HTS_Activity
Name: Counterscreen for inhibitors of the Steroid Receptor Coactivator 1 (SRC1; NCOA1): Lum...
Source: The Scripps Research Institute Molecular Screening Center
Target: transactivating tegument protein VP16 [Human herpesvirus 1]
External Id: VP16_INH_LUMI_1536_3X%INH CSRUN for SRC1
Name: Primary Screen Inhibitors of CD40 Signaling in BL2 Cells Measured in Cell-Based Syste...
Source: Broad Institute
Target: N/A
External Id: 7124-01_Inhibitor_SinglePoint_HTS_Activity
Name: Fluorescence-based cell-based primary high throughput screening assay to identify pos...
Source: The Scripps Research Institute Molecular Screening Center
Target: muscarinic acetylcholine receptor M1 [Homo sapiens]
External Id: CHRM1_PAM_FLUO8_1536_1X%ACT PRUN
Name: Luminescence-based cell-based high throughput confirmation assay for inhibitors of th...
Source: The Scripps Research Institute Molecular Screening Center
Target: nuclear receptor coactivator 1 isoform 1 [Homo sapiens]
External Id: SRC1_INH_LUMI_1536_3X%INH CRUN
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 Synonyms

Methylene violet 3RAX
3-amino-7-N,N-diethylamino-2-methyl-5-phenyl-phenazinium chloride
MFCD00013433
3-amino-7-diethylamino-5-phenyl-phenazinium,chloride
methylene violet
N,N-Diethylphenosafranine
3-Amino-7-diaethylamino-5-phenyl-phenazinium,Chlorid
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