Calcein Blue
Calcein Blue structure
|
Common Name | Calcein Blue | ||
|---|---|---|---|---|
| CAS Number | 54375-47-2 | Molecular Weight | 321.282 | |
| Density | 1.5±0.1 g/cm3 | Boiling Point | 636.2±55.0 °C at 760 mmHg | |
| Molecular Formula | C15H15NO7 | Melting Point | N/A | |
| MSDS | USA | Flash Point | 338.5±31.5 °C | |
| Symbol |
GHS07 |
Signal Word | Warning | |
Use of Calcein BlueCalcein Blue is a short-term, blue-fluorescent dye for labeling live cells. |
This table lists Chinese names, IUPAC names and various aliases of this chemical substance.
| Name | 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid |
|---|---|
| Synonym | More Synonyms |
This table contains bioactivity, target information and biological‑assay experimental data of this compound.
| Description | Calcein Blue is a short-term, blue-fluorescent dye for labeling live cells. |
|---|---|
| Related Catalog | |
| In Vitro | Calcein blue AM is only weakly fluorescent (excitation/emission maxima 322/435 nm). Upon cleavage of the AM esters by intracellular esterases, this tracer becomes relatively polar and is retained by cells for several hours. In addition, its fluorescence intensity increases and its fluorescence spectra shift to longer wavelengths, with excitation/emission maxima of 360/449 nm. The fluorescence of Calcein Blue is known to be quenched in the presence of iron(III); if a stronger chelator removes this ion from the fluorophore, the fluorescence of the fluorophore is regained[1]. A novel fluorimetric assay for dopamine using calcein blue (CB) complexed with Fe2 ion as a chemical sensor is described. The fluorescence arising from calcein blue of the calcein blue–Fe2 complex is quenched by the Fe2 ion. When dopamine is added to a solution of the calcein blue–Fe2 complex, a dopamine–Fe2 complex is formed as the result of a ligand exchange reaction between calcein blue and dopamine which permits the fluorescence from calcein blue to be recovered. The fluorescence intensity at the wavelength of 440 nm (at the excitation wavelength of 340 nm) is found to be proportional to the concentration of the dopamine added to the calcein blue–Fe2 complex solution, which permits dopamine to be quantitatively determined[2]. |
| Cell Assay | The recommended final working concentrations is usually between 1 and 10 μM to minimize potential artifacts. Generally, 15 minutes to 1 hour is sufficient for cellular uptake and processing of the dyes. A stock concentration of 5 mM 4-methylumbelliferone-8- methyliminodiacetic acid, commonly known as calcein blue is prepared in 0.1 M potassium hydroxide, and the pH is neutralized using 0.1 N hydrochloric acid. The calcein blue stock prepared is diluted to 200 μM (working stock) in DPBS and stored for further use. A concentration of 10 μM calcein blue is prepared from the working stock by diluting in DPBS. From this, 200 μL is added to a 96-well plate and the excitation/emission (Ex./Em.) maximum is determined using the micro-plate reader[1]. |
| References |
This table shows physicochemical parameters including melting point, boiling point, density, solubility and optical rotation. Missing data is marked as N/A.
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Boiling Point | 636.2±55.0 °C at 760 mmHg |
| Molecular Formula | C15H15NO7 |
| Molecular Weight | 321.282 |
| Flash Point | 338.5±31.5 °C |
| Exact Mass | 321.084839 |
| PSA | 128.28000 |
| LogP | 1.65 |
| Vapour Pressure | 0.0±2.0 mmHg at 25°C |
| Index of Refraction | 1.642 |
| InChIKey | RJPSHDMGSVVHFA-UHFFFAOYSA-N |
| SMILES | Cc1cc(=O)oc2c(CN(CC(=O)O)CC(=O)O)c(O)ccc12 |
| Storage condition | -20℃ |
This table provides MSDS information including hazard classification, first‑aid, fire‑fighting, spill handling, operation and storage instructions.
This table covers safety warnings, protective measures, incompatible substances and disposal guidelines for this compound.
| Symbol |
GHS07 |
|---|---|
| Signal Word | Warning |
| Hazard Statements | H315-H319-H335 |
| Precautionary Statements | P261-P305 + P351 + P338 |
| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Gloves |
| Hazard Codes | Xi |
| Risk Phrases | R36/37/38 |
| Safety Phrases | S26-S36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| HS Code | 2934999090 |
This table shows customs‑related data including HS‑code, tariff and regulatory conditions for import and export.
| HS Code | 2934999090 |
|---|---|
| Summary | 2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0% |
This table lists relevant public references with title, journal and publication metadata for this compound.
|
The response of anosteocytic bone to controlled loading.
J. Exp. Biol. 218 , 3559-69, (2015) The bones of the skeleton of most advanced teleost fish do not contain osteocytes. Considering the pivotal role assigned to osteocytes in the process of modeling and remodeling (the adaptation of exte... |
|
|
IL-17A activates ERK1/2 and enhances differentiation of oligodendrocyte progenitor cells.
Glia 63(5) , 768-79, (2015) Inflammatory signals present in demyelinated multiple sclerosis lesions affect the reparative remyelination process conducted by oligodendrocyte progenitor cells (OPCs). Interferon-γ (IFN-γ), tumor ne... |
|
|
FOXP3+ T Cells Recruited to Sites of Sterile Skeletal Muscle Injury Regulate the Fate of Satellite Cells and Guide Effective Tissue Regeneration.
PLoS ONE 10 , e0128094, (2015) Muscle injury induces a classical inflammatory response in which cells of the innate immune system rapidly invade the tissue. Macrophages are prominently involved in this response and required for pro... |
This table collects all English aliases, systematic names and CAS‑related synonyms of the compound.
| 2,2'-{[(7-Hydroxy-4-methyl-2-oxo-2H-chromen-8-yl)methyl]imino}diacetic acid |
| 4-Methylumbelliferone-8-methyleneiminodiacetic Acid |
| Calcein blue |
| 4-methylumbelliferone-8-methylleneiminodiacetic acid |
| MFCD00006867 |
| Umbelliferone Complexon |
| EINECS 259-129-8 |
This section contains frequently‑asked‑questions about this compound, including basic parameters, properties, storage conditions and corresponding answers.
| Q: What is the polar surface area (PSA) of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid? |
| A: Polar surface area (PSA) of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid is 128.28000. |
| Q: What is the English name of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid? |
| A: The English name of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid is 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid. |
| Q: What is the boiling point of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid? |
| A: Boiling point of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid is 636.2±55.0 °C at 760 mmHg. |
| Q: What is the safety information for 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid? |
| A: Safety information for 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid: S26-S36. |
| Q: What is the molecular weight of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid? |
| A: Molecular weight of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid is 321.282. |
| Q: What are the uses of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid? |
| A: Main uses of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid: Calcein Blue is a short-term, blue-fluorescent dye for labeling live cells. |
| Q: What are the storage conditions for 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid? |
| A: Storage conditions for 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid: -20℃. |
| Q: What English synonyms does 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid have? |
| A: English synonyms of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid: 2,2'-{[(7-Hydroxy-4-methyl-2-oxo-2H-chromen-8-yl)methyl]imino}diacetic acid, 4-Methylumbelliferone-8-methyleneiminodiacetic Acid, Calcein blue, 4-methylumbelliferone-8-methylleneiminodiacetic acid, MFCD00006867, Umbelliferone Complexon, EINECS 259-129-8. |
| Q: What is the SMILES notation of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid? |
| A: SMILES notation of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid is Cc1cc(=O)oc2c(CN(CC(=O)O)CC(=O)O)c(O)ccc12. |
| Q: What is the CAS number of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid? |
| A: CAS number of 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid is 54375-47-2. |
This table lists manufacturers and suppliers of this compound, including vendor names and product supply reference information.
| Shanghai Nianxing Industrial Co., Ltd |
| Dayang Chem (Hangzhou) Co., Ltd. |