7-Hydroxymethotrexate

Modify Date: 2024-01-01 19:49:47

7-Hydroxymethotrexate structure	Common Name	7-Hydroxymethotrexate
	CAS Number	5939-37-7	Molecular Weight	470.43900
	Density	1.68g/cm3	Boiling Point	N/A
	Molecular Formula	C₂₀H₂₂N₈O₆	Melting Point	230ºC dec.
	MSDS	N/A	Flash Point	N/A

Use of 7-Hydroxymethotrexate

7-Hydroxymethotrexate is a major metabolite of Methotrexate (MTX; HY-14519). Methotrexate, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis[1][2].

Name	7-Hydroxy Methotrexate
Synonym	More Synonyms

Description	7-Hydroxymethotrexate is a major metabolite of Methotrexate (MTX; HY-14519). Methotrexate, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis[1][2].
Related Catalog	Research Areas >> Cancer Research Areas >> Inflammation/Immunology
In Vitro	7-Hydroxymethotrexate (7-OHMTX) has the affinity of DHFR markedly lower (>100-fold) than for Methotrexate (MTX) [1].
In Vivo	7-Hydroxymethotrexate (4 mg/kg; IV) has a terminal half-life of 97.2 min and a total clearance value of 9.6 mL/min•mg[1]. Animal Model: Male Wistar rats (270-440 g)[1] Dosage: 4 mg/kg (Pharmacokinetic Analysis) Administration: IV Result: Had a terminal half-life of 97.2 min and a total clearance value of 9.6 mL/min•mg.
References	[1]. L Fahrig, et al. Pharmacokinetics of methotrexate (MTX) and 7-hydroxymethotrexate (7-OH-MTX) in rats and evidence for the metabolism of MTX to 7-OH-MTX. Cancer Chemother Pharmacol. 1989;23(3):156-60. [2]. Ping Guo, et al. Determination of methotrexate and its major metabolite 7-hydroxymethotrexate in mouse plasma and brain tissue by liquid chromatography-tandem mass spectrometry. J Pharm Biomed Anal. 2007 Apr 11;43(5):1789-95.

Density	1.68g/cm3
Melting Point	230ºC dec.
Molecular Formula	C₂₀H₂₂N₈O₆
Molecular Weight	470.43900
Exact Mass	470.16600
PSA	230.77000
LogP	1.52730
Index of Refraction	1.76

CHEMICAL IDENTIFICATION

RTECS NUMBER :: MA1000000

CHEMICAL NAME :: L-Glutamic acid, N-(4-(((2,4-diamino-1,7-dihydro-7-oxo-6-pteridinyl)me thyl)methylamino) benzoyl)-

CAS REGISTRY NUMBER :: 5939-37-7

LAST UPDATED :: 199712

DATA ITEMS CITED :: 1

MOLECULAR FORMULA :: C20-H22-N8-O6

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 300 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: CCPHDZ Cancer Chemotherapy and Pharmacology. (Springer-Verlag New York, Inc., Service Center, 44 Hartz Way, Secaucus, NJ 07094) V.1- 1978- Volume(issue)/page/year: 37,415,1996