3(2H)-Benzoxazolepropanesulfonicacid,2-[4-(1,3-dibutyltetrahydro-4,6-dioxo-2-thioxo-5(2H)-pyrimidinylidene)-2-buten-1-ylidene]-,sodium salt (1:1) structure
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Common Name | 3(2H)-Benzoxazolepropanesulfonicacid,2-[4-(1,3-dibutyltetrahydro-4,6-dioxo-2-thioxo-5(2H)-pyrimidinylidene)-2-buten-1-ylidene]-,sodium salt (1:1) | ||
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CAS Number | 62796-23-0 | Molecular Weight | 569.66900 | |
Density | N/A | Boiling Point | N/A | |
Molecular Formula | C26H32N3NaO6S2 | Melting Point | 285ºC (dec.)(lit.) | |
MSDS | Chinese USA | Flash Point | N/A | |
Symbol |
GHS07 |
Signal Word | Warning |
Use of 3(2H)-Benzoxazolepropanesulfonicacid,2-[4-(1,3-dibutyltetrahydro-4,6-dioxo-2-thioxo-5(2H)-pyrimidinylidene)-2-buten-1-ylidene]-,sodium salt (1:1)Merocyanin 540 is a fluorescent membrane probe that selectively stains the membranes of a wide variety of electrically excitable cells, but not those of nonexcitable cells (Ex/Em: 540/580 nm)[1][2]. |
Name | merocyanine 540 |
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Synonym | More Synonyms |
Description | Merocyanin 540 is a fluorescent membrane probe that selectively stains the membranes of a wide variety of electrically excitable cells, but not those of nonexcitable cells (Ex/Em: 540/580 nm)[1][2]. |
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Related Catalog | |
In Vitro | Guidelines[1] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Cells: 1. Wash monolayer cultured cells with isotonic saline with or without 1-5 mM CaCl2 or iso-osmotic sucrose (0.25 M) solutions buffered with 5 mM Tris-Cl (pH 7.4). 2. Expose cells to Merocyanin 540 (1.6 x 10-5 M) in the appropriate buffer for 10 min at room temperature under ambient lighting conditions. 3. Wash extensively and examine by fluorescence microscopy. 4. For protozoa, place 0.05 mL aliquots of washed cultures containing 100-200 organisms on a microscope slide. 5. Add Merocyanin 540 to a final concentration of 1.0-10 μg/mL. 6. Examine cells by fluorescence microscopy. 7. Incubate single cell suspensions or tissue slices with Merocyanin 540 (1.75-5.25 x 10-5 M) and examine by fluorescence microscopy as described for attached cells. |
References |
Melting Point | 285ºC (dec.)(lit.) |
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Molecular Formula | C26H32N3NaO6S2 |
Molecular Weight | 569.66900 |
Exact Mass | 569.16300 |
PSA | 156.36000 |
LogP | 4.22630 |
Storage condition | 2-8°C |
CHEMICAL IDENTIFICATION
HEALTH HAZARD DATAACUTE TOXICITY DATA
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Symbol |
GHS07 |
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Signal Word | Warning |
Hazard Statements | H315-H319-H335 |
Precautionary Statements | P261-P305 + P351 + P338 |
Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Gloves |
Hazard Codes | Xi |
Risk Phrases | 36/37/38 |
Safety Phrases | S26;S36 |
RIDADR | NONH for all modes of transport |
WGK Germany | 3 |
RTECS | DM4866500 |
Fluorescence ratiometric properties induced by nanoparticle plasmonics and nanoscale dye dynamics.
ScientificWorldJournal 2013 , 624505, (2013) Nanoscale transport of merocyanine 540 within/near the plasmon field of gold nanoparticles was recognized as an effective inducer of single-excitation dual-emission ratiometric properties. With a high... |
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Acrosin activity is a good predictor of boar sperm freezability.
Theriogenology 83 , 1525-33, (2015) The main aim of this study was to determine whether acrosin activity could predict boar sperm freezability. For this purpose, we characterized the changes in sperm quality and acrosin activity through... |
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B cell development in the spleen takes place in discrete steps and is determined by the quality of B cell receptor-derived signals.
J. Exp. Med. 190 , 75-89, (1999) Only mature B lymphocytes can enter the lymphoid follicles of spleen and lymph nodes and thus efficiently participate in the immune response. Mature, long-lived B lymphocytes derive from short-lived p... |
MEROCYANINE 540 |
2-[4-(1,3-Dibutyl-1,3,4,6-tetrahydro-4,6-dioxo-2-thioxopyrimidin-5(2H)-ylidene)-2-butenylidene]-3(2H)-benzoxazolepropane-1-sulfonic acid sodium salt |
MFCD00013438 |
2-(4-(1,3-dibutyltetrahydro-4,6-dioxo-2-3(2h)-benzoxazolepropanesulfonicaci |
Merocyanin 540 |