DiSBAC2(5)

Modify Date: 2024-01-02 19:05:57

DiSBAC2(5) Structure
DiSBAC2(5) structure
Common Name DiSBAC2(5)
CAS Number 70363-83-6 Molecular Weight 516.63
Density N/A Boiling Point N/A
Molecular Formula C27H40N4O6 Melting Point N/A
MSDS USA Flash Point N/A

 Use of DiSBAC2(5)


DiBAC4(3) is a voltage-sensitive fluorescent dye (λex=490 nm, λem=505 nm).

 Names

Name Bis(1,3-dibutylbarbituric acid) trimethine oxonol
Synonym More Synonyms

 DiSBAC2(5) Biological Activity

Description DiBAC4(3) is a voltage-sensitive fluorescent dye (λex=490 nm, λem=505 nm).
Related Catalog
In Vitro The membrane hyperpolarization induced by 10 μM Evans blue (EB) in HEKBKα is clearly observed with DiBAC4(3), while the change in membrane potential (MP) by addition of 3 mM tetraethylammonium chloride (TEA) appears more slowly than that measured with microelectrode. The time to peak of hyperpolarization is 2.3±0.9 s (n=4) and 35.0±2.6 s (n=12, P<0.01) by the measurements with microelectrodes and DiBAC4(3), respectively[1].
Cell Assay Prior to the fluorescence measurements, cells are incubated in KRH (Krebs-Ringer-HEPES) buffer containing with 100 nM DiBAC4(3) for 20 min at room temperature. The stained cells are used for experiments without washing. The fluorescence emission is collected using a 505 nm dicroic mirror and a BA filter (>520 nm)[1].
References

[1]. Yamada A, et al. Usefulness and limitation of DiBAC4(3), a voltage-sensitive fluorescent dye, for the measurement of membrane potentials regulated by recombinant large conductance Ca2+-activated K+ channels in HEK293 cells. Jpn J Pharmacol. 2001 Jul;86(3):342-50.

 Chemical & Physical Properties

Molecular Formula C27H40N4O6
Molecular Weight 516.63
PSA 200.30000
LogP 2.90160
Storage condition 2~8°C

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes Xn
RIDADR NONH for all modes of transport

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 Synonyms

dibac4(3)
MFCD00467633