Bromothymol blue
Bromothymol blue structure
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Common Name | Bromothymol blue | ||
|---|---|---|---|---|
| CAS Number | 76-59-5 | Molecular Weight | 624.381 | |
| Density | 1.5±0.1 g/cm3 | Boiling Point | 614.3±55.0 °C at 760 mmHg | |
| Molecular Formula | C27H28Br2O5S | Melting Point | 204 °C | |
| MSDS | Chinese USA | Flash Point | 325.3±31.5 °C | |
| Symbol |
GHS02 |
Signal Word | Warning | |
Use of Bromothymol blueBromothymol Blue is a pH indicator. |
This table lists Chinese names, IUPAC names and various aliases of this chemical substance.
| Name | bromothymol blue |
|---|---|
| Synonym | More Synonyms |
This table contains bioactivity, target information and biological‑assay experimental data of this compound.
| Description | Bromothymol Blue is a pH indicator. |
|---|---|
| Related Catalog | |
| In Vitro | A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer. A pH-shift assay is developed on the basis of BTB as the pH indicator and potassium phosphate as the buffer component[1]. Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate. The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5). Upon the addition of a Bromothymol Blue solution (0.1%, w/v) to an agar plate, zones surrounding colonies of K. vulgare 07 mutants change their color from blue to yellow because K. vulgare 07 mutants release 2-KLG on agar plates, thereby acidifying surrounding areas around colonies[2]. |
| Kinase Assay | For all three enzymes, an expression in the 96-deep well scale is performed. Therefore, electrocompetent E. coli ArcticExpress(DE3) cells are transformed with the corresponding plasmid. Single clones are picked using the CP7200 Colony Picker and transferred to 96-deep well plates filled with 1.2 mL autoinduction media by a MicroFlo Select dispenser. After incubation (36 h, 37°C at 1,000 rpm), further processing is done manually. First, 100 μL of cell culture is transferred into a 96-well plate (U-shaped bottom) and harvested by centrifugation (4,570 rpm, 10 min at RT) while the supernatant is discarded. The frozen pellets (1 h at -20°C) are thawed at room temperature for one hour to improve cell lysis. Lysis is continued by the addition of 30 μL lysis buffer (3 h, 1,000 rpm, 37°C) containing 2 mM KPi, pH 7.0, 2 mM MgCl2, 10 μg/mL DNaseI, 100 μg/mL lysozyme. Next, 120 μL buffer (2 mM KPi, pH 7.0) is added followed by centrifugation (3,000 rpm, 15 min at RT). For the photometric measurement, 20 μL of the crude extract is transferred to a 96-well plate (F-shaped bottom) and the reaction is started by adding 180 μL master mix to give a final volume of 200 μL (2.5 mM KPi, pH 7.0, 2 mM MgCl2, 25 μg/mL BTB and 5 mM keto-deoxy-D-glucarate). The measurements are carried out for 60 min at 2-min intervals. Depending on the enzyme, different time windows are used for the activity calculation[1]. |
| References |
This table shows physicochemical parameters including melting point, boiling point, density, solubility and optical rotation. Missing data is marked as N/A.
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Boiling Point | 614.3±55.0 °C at 760 mmHg |
| Melting Point | 204 °C |
| Molecular Formula | C27H28Br2O5S |
| Molecular Weight | 624.381 |
| Flash Point | 325.3±31.5 °C |
| Exact Mass | 622.002380 |
| PSA | 92.21000 |
| LogP | 8.60 |
| Vapour Pressure | 0.0±1.8 mmHg at 25°C |
| Index of Refraction | 1.634 |
| InChIKey | NUHCTOLBWMJMLX-UHFFFAOYSA-N |
| SMILES | Cc1c(C2(c3cc(C(C)C)c(O)c(Br)c3C)OS(=O)(=O)c3ccccc32)cc(C(C)C)c(O)c1Br |
| Storage condition | Store at RT. |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| Water Solubility | slightly soluble |
This table provides MSDS information including hazard classification, first‑aid, fire‑fighting, spill handling, operation and storage instructions.
This table summarizes toxicological test data, acute & chronic toxicity and ecological hazard parameters for this chemical.
CHEMICAL IDENTIFICATION
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This table covers safety warnings, protective measures, incompatible substances and disposal guidelines for this compound.
| Symbol |
GHS02 |
|---|---|
| Signal Word | Warning |
| Hazard Statements | H226 |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
| Hazard Codes | Xn |
| Risk Phrases | R10 |
| Safety Phrases | S24/25-S39-S26 |
| RIDADR | NONH for all modes of transport |
| RTECS | SJ450000 |
| Packaging Group | III |
| HS Code | 29349990 |
This table presents publicly reported synthetic routes, reaction conditions, reactants and product information of the compound.
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~%
Bromothymol blue CAS#:76-59-5 |
| Literature: J. Washington Acad., vol. 6, p. 482 Chem. Zentralbl., , vol. 87, # II p. 1068 J. Washington Acad., vol. 5, p. 616 Chem. Zentralbl., , vol. 87, # I p. 175 |
|
~%
Bromothymol blue CAS#:76-59-5 |
| Literature: J. Washington Acad., vol. 6, p. 482 Chem. Zentralbl., , vol. 87, # II p. 1068 |
|
~%
Bromothymol blue CAS#:76-59-5 |
| Literature: J. Washington Acad., vol. 6, p. 482 Chem. Zentralbl., , vol. 87, # II p. 1068 J. Washington Acad., vol. 5, p. 616 Chem. Zentralbl., , vol. 87, # I p. 175 |
This table lists upstream starting materials and downstream derivative products related to this chemical.
| Precursor 4 | |
|---|---|
| DownStream 0 | |
This table shows customs‑related data including HS‑code, tariff and regulatory conditions for import and export.
| HS Code | 29349990 |
|---|
This table lists relevant public references with title, journal and publication metadata for this compound.
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Real-time UV imaging identifies the role of pH in insulin dissolution behavior in hydrogel-based subcutaneous tissue surrogate.
Eur. J. Pharm. Sci. 69 , 26-36, (2015) For parenteral biopharmaceuticals, subcutaneous diffusion and, in the case of solid implants or suspensions, dissolution may govern the clinical profile of the drug product. Insight into the dissoluti... |
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The transcription factor BcLTF1 regulates virulence and light responses in the necrotrophic plant pathogen Botrytis cinerea.
PLoS Genet. 10(1) , e1004040, (2014) Botrytis cinerea is the causal agent of gray mold diseases in a range of dicotyledonous plant species. The fungus can reproduce asexually by forming macroconidia for dispersal and sclerotia for surviv... |
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The VELVET Complex in the Gray Mold Fungus Botrytis cinerea: Impact of BcLAE1 on Differentiation, Secondary Metabolism, and Virulence.
Mol. Plant Microbe Interact. 28 , 659-74, (2015) Botrytis cinerea, the gray mold fungus, is an important plant pathogen. Field populations are characterized by variability with regard to morphology, the mode of reproduction (conidiation or sclerotia... |
This table contains target information, in‑vitro & in‑vivo assay data for this compound.
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Name: qHTS Assay for Substrates of Mammalian Selenoprotein Thioredoxin Reductase 1 (TrxR1):...
Source: NCGC
Target: thioredoxin reductase [Rattus norvegicus]
External Id: TRXR101
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Name: qHTS for Inhibitors of Polymerase Iota
Source: NCGC
Target: DNA polymerase iota [Homo sapiens]
External Id: PolI100
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Name: qHTS for Inhibitors of human tyrosyl-DNA phosphodiesterase 1 (TDP1): qHTS in cells in...
Source: NCGC
Target: TDP1 protein [Homo sapiens]
External Id: TDP1100
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Name: qHTS for Stage-Specific Inhibitors of Vaccinia Orthopoxvirus: mCherry Reporter Primar...
Source: NCGC
Target: 67.9K protein [Vaccinia virus]
External Id: Vaccinia-p2mCherry
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Name: qHTS for Inhibitors of human tyrosyl-DNA phosphodiesterase 1 (TDP1): qHTS in cells in...
Source: NCGC
Target: TDP1 protein [Homo sapiens]
External Id: TDP1101
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Name: qHTS for Inhibitors of Polymerase Kappa
Source: NCGC
Target: DNA polymerase kappa [Homo sapiens]
External Id: PolK100
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Name: qHTS Assay for Inhibitors of Mammalian Selenoprotein Thioredoxin Reductase 1 (TrxR1):...
Source: NCGC
Target: thioredoxin reductase [Rattus norvegicus]
External Id: TRXR100
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Name: qHTS for Stage-Specific Inhibitors of Vaccinia Orthopoxvirus: Venus Reporter Primary ...
Source: NCGC
Target: 67.9K protein [Vaccinia virus]
External Id: Vaccinia-p2Venus
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Name: qHTS for Inhibitors of TGF-b: Cytotox Counterscreen
Source: NCGC
Target: N/A
External Id: SMAD3201
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This table collects all English aliases, systematic names and CAS‑related synonyms of the compound.
| 2-bromo-4-[3-(3-bromo-4-hydroxy-2-methyl-5-propan-2-ylphenyl)-1,1-dioxo-2,1λ<sup>6</sup>-benzoxathiol-3-yl]-3-methyl-6-propan-2-ylphenol |
| Phenol, 4,4'-(3H-2,1-benzoxathiol-3-ylidene)bis[2-bromo-3-methyl-6-(1-methylethyl)-, S,S-dioxide |
| EINECS 200-971-2 |
| a,a-Bis(6-bromo-5-hydroxycarvacryl)-a-hydroxy-o-toluenesulfonic Acid g-Sultone |
| 4,4'-(1,1-dioxido-3H-2,1-benzoxathiole-3,3-diyl)bis[2-bromo-3-methyl-6-(propan-2-yl)phenol] |
| Thymol, 6,6'-(3H-2,1-benzoxathiol-3-ylidene)bis(2-bromo-, S,S-dioxide (8CI) |
| 4,4'-(3H-2,1-Benzoxathiol-3-ylidene)bis[2-bromo-3-methyl-6-(1-methylethyl)phenol] S,S-Dioxide |
| 3',3''-Dibromothymolsulfonphthalein |
| Bromthymol Blue |
| 4,4'-(1,1-Dioxido-3H-2,1-benzoxathiole-3,3-diyl)bis(2-bromo-6-isopropyl-3-methylphenol) |
| Bromothymol blue |
| MFCD00005872 |
This section contains frequently‑asked‑questions about this compound, including basic parameters, properties, storage conditions and corresponding answers.
| Q: What are the upstream materials of bromothymol blue? |
| A: Related upstream materials(CAS) of bromothymol blue: 76-61-9;89-83-8;7726-95-6;64-19-7. |
| Q: What are the storage conditions for bromothymol blue? |
| A: Storage conditions for bromothymol blue: Store at RT. |
| Q: What is the melting point of bromothymol blue? |
| A: Melting point of bromothymol blue is 204 °C. |
| Q: What is the SMILES notation of bromothymol blue? |
| A: SMILES notation of bromothymol blue is Cc1c(C2(c3cc(C(C)C)c(O)c(Br)c3C)OS(=O)(=O)c3ccccc32)cc(C(C)C)c(O)c1Br. |
| Q: What is the polar surface area (PSA) of bromothymol blue? |
| A: Polar surface area (PSA) of bromothymol blue is 92.21000. |
| Q: What are the uses of bromothymol blue? |
| A: Main uses of bromothymol blue: Bromothymol Blue is a pH indicator. |
| Q: What is the LogP of bromothymol blue? |
| A: LogP of bromothymol blue is 8.60. |
| Q: What is the CAS number of bromothymol blue? |
| A: CAS number of bromothymol blue is 76-59-5. |
| Q: What is the boiling point of bromothymol blue? |
| A: Boiling point of bromothymol blue is 614.3±55.0 °C at 760 mmHg. |
| Q: What is the safety information for bromothymol blue? |
| A: Safety information for bromothymol blue: S24/25-S39-S26. |
This table lists manufacturers and suppliers of this compound, including vendor names and product supply reference information.
| Shanghai Nianxing Industrial Co., Ltd |
| Dayang Chem (Hangzhou) Co., Ltd. |
| Henan Tianfu Chemical Co., Ltd. |