7-Ethyl-10-hydroxycamptothecin

Modify Date: 2024-01-01 20:18:45

7-Ethyl-10-hydroxycamptothecin structure	Common Name	7-Ethyl-10-hydroxycamptothecin
	CAS Number	86639-52-3	Molecular Weight	392.405
	Density	1.5±0.1 g/cm3	Boiling Point	810.3±65.0 °C at 760 mmHg
	Molecular Formula	C₂₂H₂₀N₂O₅	Melting Point	217 °C
	MSDS	Chinese USA	Flash Point	443.8±34.3 °C
	Symbol	GHS06	Signal Word	Danger

Use of 7-Ethyl-10-hydroxycamptothecin

SN-38 is an active metabolite of the Topoisomerase I inhibitor Irinotecan. SN-38 inhibits DNA and RNA synthesis with IC50s of 0.077 and 1.3 μM, respectively.

Name	7-Ethyl-10-hydroxycamptothecin
Synonym	More Synonyms

Description	SN-38 is an active metabolite of the Topoisomerase I inhibitor Irinotecan. SN-38 inhibits DNA and RNA synthesis with IC50s of 0.077 and 1.3 μM, respectively.
Related Catalog	Signaling Pathways >> Autophagy >> Autophagy Signaling Pathways >> Cell Cycle/DNA Damage >> Topoisomerase Research Areas >> Cancer
Target	Topoisomerase I
In Vitro	The IC50 values for LoVo, HCT116, and HT29 cell lines is 20 nM, 50 nM, 130 nM, respectively. In all three SN-38 resistant cell lines Top1 activity is maintained in the presence of high concentrations of SN-38[2].
In Vivo	SN-38, the active and toxic metabolite of the anticancer prodrug Irinotecan. At 30 minutes after administration, Irinotecan plasma concentrations in Slco1a/1b(−/−) mice are 1.9-fold higher than in the wild-type mice (1.89 vs. 1.01 μM, respectively), whereas SN-38 plasma concentrations of Slco1a/1b(−/−) mice are 8-fold higher compare with wild-type mice (0.4 μg/mL vs. 0.05 μg/mL, respectively). Overall plasma exposure [AUC(5-240)] of Irinotecan is 1.7-fold higher in Oatp1a/1b knockout mice versus wild-type mice (209.8±6.7 vs. 120.9±4.4 μM/min; P<0.01), and 2.9-fold higher for SN-38 (50±2.9 vs. 12±2 μM/min; P<0.001)[3].
Kinase Assay	LoVo, HCT116, and HT29 cell lines are trypsinized, resuspended and counted, and for each cell line 1 million cells are pipetted to each of three eppendorf tubes on ice. Cells are pelleted (5 min centrifugation, 300 g, 4°C) and snap-frozen in dry ice and ethanol and stored at -80°C until analysis. Nuclear extracts are prepared essentially, and Top1 activity measured in titration experiments with or without added SN-38 (at concentrations stated in the text) using the standard Rolling circle Enhanced Enzyme Activity Detection (REEAD) protocol. The activity is calculated in terms of numbers of Top1 specific signals relative to the amount of signals resulting from the addition a known concentration of control circles[2].
Cell Assay	In vitro SN-38 sensitivity is determined using the MTT assay. Cells are seeded in 96-well plates, and a range of SN-38 concentrations is added the following day. Following 48 h of drug exposure, the medium is discarded and the plates are incubated with medium containing MTT (0.5 mg/mL) for 3 h. Acidified (0.02 M HCl) sodium dodecyl sulphate (20 %) is added to dissolve the formed formazan. Optical density at 570 nm (and 670 nm for background) is measured, and the cell viability is calculated in percent compared to untreated cells. Experiments are repeated three times and the mean IC50 value ± standard deviation is determined. Relative resistance for each resistant cell line is calculated by dividing the mean IC50 value of the resistant cell line by the mean IC50 value of the corresponding parental cell line[2].
Animal Admin	Mice[3] Female wild-type, Slco1a/1b(−/−)(Oatp1a/1b knockout), Slco1a/1b(−/−);1B1(tg), and Slco1a/1b(−/−);1B3(tg) (liver-specific OATP1B1 and OATP1B3 humanized transgenic) mice of comparable genetic background (>99% FVB) between 8 and 14 weeks of age are used. Irinotecan (20 mg/mL in water-based solution containing NaOH, lactic acid, and sorbitol) is diluted with saline (to 2 mg/mL) for administration of 10 mg/kg; 5 μL/g bodyweight are administered intravenously to mice. SN-38 is dissolved in DMSO (1 mg/mL) and 1 μL/g body weight is administered intravenously to mice to achieve a dosage of 1 mg/kg. The experiments are terminated by isoflurane anaesthesia, heparin-blood sampling by cardiac puncture followed by cervical dislocation and tissue collection. Blood samples are centrifuged at 5,200 × g for 5 minutes at 4°C and plasma is collected and stored at −30°C until analysis.
References	[1]. Wallin A, et al. Anticancer effect of SN-38 on colon cancer cell lines with different metastatic potential. Oncol Rep. 2008 Jun;19(6):1493-8. [2]. Jensen NF, et al. Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a newpair of resistance-associated mutations. J Exp Clin Cancer Res. 2016 Mar 31;35:56. [3]. Stewart CF, et al. Disposition of irinotecan and SN-38 following oral and intravenous irinotecan dosing in mice. Cancer Chemother Pharmacol. 1997;40(3):259-65.

Density	1.5±0.1 g/cm3
Boiling Point	810.3±65.0 °C at 760 mmHg
Melting Point	217 °C
Molecular Formula	C₂₂H₂₀N₂O₅
Molecular Weight	392.405
Flash Point	443.8±34.3 °C
Exact Mass	392.137207
PSA	101.65000
LogP	2.31
Vapour Pressure	0.0±3.0 mmHg at 25°C
Index of Refraction	1.738
Storage condition	−20°C

7-Ethyl-10-hydroxycamptothecin MSDS(Chinese)

CHEMICAL IDENTIFICATION

RTECS NUMBER :: UQ0491000

CAS REGISTRY NUMBER :: 86639-52-3

LAST UPDATED :: 199203

DATA ITEMS CITED :: 2

MOLECULAR FORMULA :: C22-H20-N2-O5

MOLECULAR WEIGHT :: 392.44

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

MUTATION DATA

TYPE OF TEST :: DNA inhibition

TEST SYSTEM :: Rodent - mouse Leukocyte

DOSE/DURATION :: 1 umol/L

REFERENCE :: CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut Sts., Philadelphia, PA 19106) V.1- 1941- Volume(issue)/page/year: 51,4187,1991

Symbol	GHS06
Signal Word	Danger
Hazard Statements	H301
Precautionary Statements	P301 + P310
Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	T
Risk Phrases	25
Safety Phrases	45
RIDADR	UN 2811 6.1 / PGIII
RTECS	UQ0491000
HS Code	2942000000

Precursor 8
CAS#:78287-27-1 7-Ethylcamptothecin CAS#:35364-15-9 1-(2-Amino-5-hy... CAS#:110351-94-5 (S)-4-ethyl-4-h... CAS#:797762-11-9 7-ethyl-1,2,6,7...
CAS#:100286-90-6 Irinotecan hydr... CAS#:19685-09-7 (S)-10-Hydroxyc... CAS#:123-38-6 Propionaldehyde CAS#:102978-40-5 (±)- 1H-Pyrano[...
DownStream 4
CAS#:97682-44-5 Irinotecan CAS#:121080-63-5 SN-38 GLUCURONIDE CAS#:100286-90-6 Irinotecan hydr... CAS#:121098-77-9 SN-38 Tri-O-ace...

HS Code	2942000000

Evaluation of the in vitro/in vivo potential of five berries (bilberry, blueberry, cranberry, elderberry, and raspberry ketones) commonly used as herbal supplements to inhibit uridine diphospho-glucuronosyltransferase.

Food Chem. Toxicol. 72 , 13-9, (2014)

In this study, we evaluated inhibitory potentials of popularly-consumed berries (bilberry, blueberry, cranberry, elderberry, and raspberry ketones) as herbal supplements on UGT1A1, UGT1A4, UGT1A6, UGT...

Evaluation of thein vitro/in vivodrug interaction potential of BST204, a purified dry extract of ginseng, and its four bioactive ginsenosides through cytochrome P450 inhibition/induction and UDP-glucuronosyltransferase inhibition

Food Chem. Toxicol. 68 , 117-27, (2014)

• BST204 is a purified dry extract of ginseng containing high amounts of Rh2 and Rg3. • BST204 had only weak inhibitory effects on nine CYPs and five UGTs. • It is unlikely that BST204 alter pharmacok...

Nanoparticle delivery of an SN38 conjugate is more effective than irinotecan in a mouse model of neuroblastoma.

Cancer Lett. 360(2) , 205-12, (2015)

Neuroblastoma (NB) is the most common and deadly solid tumor in children. The majority of NB patients have advanced stage disease with poor prognosis, so more effective, less toxic therapy is needed. ...

7-Ethyl-10-Hydroxycamptothecin(SN-38)

(4S)-4,11-Diethyl-4,9-dihydroxy-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione

1H-Pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione, 4,11-diethyl-4,9-dihydroxy-, (4S)-

SN-38

MFCD06762720

SN38

7-Ethyl-10-hydroxycamptothecine

Shanghai Nianxing Industrial Co., Ltd
China
Product Name: 7-Ethyl-10-hydroxycamptothecin
Price: Click To Check
Purity: 98.0%
Delivery: 10 Day
Contact: Yang
Email: sales@echemcloud.com

BioBioPha
China
Product Name: 7-Ethyl-10-hydroxycamptothecin
Price: ￥Inquiry/5mg
Purity: 98.0%
Delivery: 10 Day
Contact: Xueping-Zheng
Email: 1477035482@qq.com

Shanghai Jizhi Biochemical Technology Co., Ltd
China
Product Name: 7-Ethyl-10-hydroxycamptothecin
Price: ￥498.0/20mg ￥658.0/1g
Purity: 98.0%
Delivery: 2 Day
Contact: Liu jia
Email: 2130147988@qq.com

DC Chemicals Limited
China
Product Name: SN-38(NK-012)
Price: $300.0/1g $150.0/10ml
Purity: 98.0%
Delivery: 3 Day
Contact: Tony Cao
Email: sales@dcchemicals.com

ShangHai DC Chemicals Co.,Ltd
China
Product Name: SN-38(NK-012)
Price: ￥Inquiry/1g
Purity: 98.9%
Delivery: 1 Day
Contact: Tony Lai
Email: order@dcchemicals.com

Dayang Chem (Hangzhou) Co., Ltd.
China
Product Name: 7-Ethyl-10-hydroxycamptothecin
Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
Purity: 98.0%
Delivery: Inquiry
Contact: Ms Wang
Email: enquiry@dycnchem.com

Henan Tianfu Chemical Co., Ltd.
China
Product Name: 7-Ethyl-10-hydroxycamptothecin
Price: $Inquiry/1kg $Inquiry/25kg $Inquiry/1000kg $Inquiry/10ton
Purity: 99.0%
Delivery: Inquiry
Contact: Anson
Email: info@tianfuchem.com

Get all suppliers and price by the below link:

7-Ethyl-10-hydroxycamptothecin suppliers

Price: ￥87.1/100mg

Reference only. check more 7-Ethyl-10-hydroxycamptothecin price

7-Ethyl-10-hydroxycamptothecin

Use of 7-Ethyl-10-hydroxycamptothecin

Names