T7 RNA polymerase

Modify Date: 2024-01-18 10:09:42

T7 RNA polymerase Structure
T7 RNA polymerase structure
Common Name T7 RNA polymerase
CAS Number 9014-24-8 Molecular Weight N/A
Density N/A Boiling Point N/A
Molecular Formula N/A Melting Point N/A
MSDS USA Flash Point N/A

 Use of T7 RNA polymerase


T7 RNA polymerase is a polymerase expressed by Escherichia coli from the RNA polymerase gene of T7 bacteriophage. T7 RNA polymerase is highly specific and involved in in vitro transcription (IVT) of mRNA. In the presence of Mg2+, T7 RNA polymerase only uses the single-stranded or double-stranded DNA containing the T7 promoter sequence as a template, and uses NTP as a substrate to synthesize RNA complementary to the single-stranded DNA downstream of the promoter[1][2].

 Names

Name RNA POLYMERASE
Synonym More Synonyms

 T7 RNA polymerase Biological Activity

Description T7 RNA polymerase is a polymerase expressed by Escherichia coli from the RNA polymerase gene of T7 bacteriophage. T7 RNA polymerase is highly specific and involved in in vitro transcription (IVT) of mRNA. In the presence of Mg2+, T7 RNA polymerase only uses the single-stranded or double-stranded DNA containing the T7 promoter sequence as a template, and uses NTP as a substrate to synthesize RNA complementary to the single-stranded DNA downstream of the promoter[1][2].
Related Catalog
References

[1]. Dousis A, et al. An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts. Nat Biotechnol. 2023 Apr;41(4):560-568.  

[2]. Borkotoky S, et al. The highly efficient T7 RNA polymerase: A wonder macromolecule in biological realm. Int J Biol Macromol. 2018 Oct 15;118(Pt A):49-56.  

 Chemical & Physical Properties

Storage condition −20°C

 Safety Information

Hazard Codes Xi
RIDADR NONH for all modes of transport
WGK Germany 3

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 Synonyms

EINECS 232-756-4