Discovery of a highly selective and efficient reagent for formation of intramolecular disulfide bonds in peptides

T Shi, DL Rabenstein

Index: Shi, Tiesheng; Rabenstein, Dallas L. Journal of the American Chemical Society, 2000 , vol. 122, # 29 p. 6809 - 6815

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Citation Number: 36

Abstract

We have discovered that trans-[Pt (en) 2Cl2] 2+(en= ethylenediamine) is a highly selective and efficient reagent for the quantitative formation of intramolecular disulfide bonds in peptides. A series of 14 dithiol peptides which form disulfide-containing rings ranging in size from 14 to 53 atoms were used to characterize the reagent. The dithiol peptides are cleanly and rapidly converted to their disulfide forms by a slight excess of the platinum complex ...