Directed evolution to investigate steric control of enzymatic oxidosqualene cyclization. An isoleucine-to-valine mutation in cycloartenol synthase allows lanosterol and …

…, L Hua, LB Darr, WK Wilson, J Pang…

Index: Hart, Elizabeth A.; Hua, Ling; Darr, Lisa B.; Wilson, William K.; Pang, Jihai; Matsuda, Seiichi P. T. Journal of the American Chemical Society, 1999 , vol. 121, # 42 p. 9887 - 9888

Full Text: HTML

Citation Number: 103

Abstract

Oxidosqualene cyclases convert (S)-2, 3-oxidosqualene to triterpene alcohols by protonation, cyclization, rearrangement, and elimination reactions. This catalytic approach has great plasticity and produces a large family of compounds; 83 different natural products are C30H50O isomers structurally consistent with being enzymatically rearranged products of oxidosqualene. 1, 2 Two rigorously conserved Saccharomyces cereVisiae lanosterol ...

Related Articles:

Conformational analysis of cycloartenol, 24-methylenecycloartanol and their derivatives

[Yoshida, Kumi; Hirose, Yoshihiko; Imai, Yutaka; Kondo, Tadao Agricultural and Biological Chemistry, 1989 , vol. 53, # 7 p. 1901 - 1912]

Genome mining for sesterterpenes using bifunctional terpene synthases reveals a unified intermediate of di/sesterterpenes

[Seo, Shujiro; Uomori, Atsuko; Yoshimura, Yohko; Takeda, Ken'ichi; Seto, Haruo; et.al. Journal of the Chemical Society, Perkin Transactions 1: Organic and Bio-Organic Chemistry (1972-1999), 1988 , p. 2407 - 2414]

Arundinol, a new triterpene from the orchid Arundina bambusifolia

[Majumder; Ghosal Journal of the Indian Chemical Society, 1991 , vol. 68, # 2 p. 88 - 91]

More Articles...