Enzyme-purification and catalytic transformations in a microstructured PASS flow reactor using a new tyrosine-based Ni-NTA linker system attached to a …
G Dräger, C Kiss, U Kunz, A Kirschning
Index: Draeger, Gerald; Kiss, Csilla; Kunz, Ulrich; Kirschning, Andreas Organic and Biomolecular Chemistry, 2007 , vol. 5, # 22 p. 3657 - 3664
Full Text: HTML
Citation Number: 43
Abstract
The synthesis of a Ni-nitrilotriacetic acid (Ni-NTA) attached via a new tyrosine-based linker matrix on monolithic crosslinked poly (vinyl benzyl chloride)/poly (vinylpyrrolidinone) is described. This matrix is incorporated inside a microstructured PASSflow reactor which was used for automatic purification and immobilisation of His6-tagged proteins. These could be used as stable and highly active biocatalysts for the synthesis of (R)-benzoin (6),(R)-2- ...
Related Articles:
[Muthupandi, Pandi; Sekar, Govindasamy Tetrahedron Letters, 2011 , vol. 52, # 6 p. 692 - 695]
[Krawczyk, Ewa; Mielniczak, Grazyna; Owsianik, Krzysztof; Luczak, Jerzy Tetrahedron Asymmetry, 2012 , vol. 23, # 20-21 p. 1480 - 1489]
[Organic and Biomolecular Chemistry, , vol. 5, # 22 p. 3657 - 3664]
[Chemistry - A European Journal, , vol. 15, # 5 p. 1086 - 1090]
[Enders, Dieter; Breuer, Klaus; Teles, J. Henrique Helvetica Chimica Acta, 1996 , vol. 79, # 4 p. 1217 - 1221]