Planta medica

Urinary excretion of arbutin metabolites after oral administration of bearberry leaf extracts

J Quintus, KA Kovar, P Link, H Hamacher

Index: Quintus, Joachim; Kovar, Karl-Artur; Link, Peter; Hamacher, Harald Planta Medica, 2005 , vol. 71, # 2 p. 147 - 152

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Citation Number: 39

Abstract

Abstract An HPLC assay with fluorimetric detection of the arbutin metabolites hydroquinone glucuronide (2) and hydroquinone sulphate (6) in urine was developed and validated. Methylarbutin (4) and 6 were synthesised as reference substances. Compound 2 was prepared enzymatically from hydroquinone and uridine 5′-diphosphoglucuronic acid using the glucosyltransferase system of rat liver microsomes and enriched by two liquid-liquid ...