FT-ICR-MS characterization of intermediates in the biosynthesis of the α-methylbutyrate side chain of lovastatin by the 277 kDa polyketide synthase LovF

…, X Xie, X Zhao, W Xu, Y Tang, PC Dorrestein

Index: Xie, Xinkai; Meehan, Michael J.; Xu, Wei; Dorrestein, Pieter C.; Tang, Yi Journal of the American Chemical Society, 2009 , vol. 131, p. 8388 - 8389

Full Text: HTML

Citation Number: 10

Abstract

There are very few fungal polyketide synthases that have been characterized by mass spectrometry. In this paper we describe the in vitro reconstitution and FT-ICR-MS verification of the full activity of an intact 277 kDa fungal polyketide synthase LovF of the lovastatin biosynthetic pathway. We report here both the verification of the reconstitution of fully functional holo-LovF by using 13C-labeled malonyl-CoA to form α-methylbutyrate ...