Tetrahedron letters

Chemoenzymatic one-pot synthesis of cefazolin from cephalosporin C in fully aqueous medium, involving three consecutive biotransformations catalyzed by D- …

R Fernández-Lafuente, JM Guisán, M Pregnolato…

Index: Fernandez-Lafuente, Roberto; Guisan, Jose M.; Pregnolato, Massimo; Terreni, Marco Tetrahedron Letters, 1997 , vol. 38, # 26 p. 4693 - 4696

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Citation Number: 25

Abstract

A new chemoenzymatic synthesis of Cefazolin through the correct assembly of three biotransformations catalysed by D-aminoacid oxidase, glutaryl acylase and penicillin G acylase is described. This multienzymatic synthesis has been performed from the natural Cephalosporin C in fully aqueous medium without intermediate purification stages. Almost quantitative yields have been achieved in all the enzymatic reactions.

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