International Journal of Biological Macromolecules 2006-11-15

Stepwise modification of lysine residues of glucose oxidase with citraconic anhydride.

Shahla Mossavarali, Saman Hosseinkhani, Bijan Ranjbar, Mehran Miroliaei

Index: Int. J. Biol. Macromol. 39(4-5) , 192-6, (2006)

Full Text: HTML

Abstract

Structural properties of modified forms of glucose oxidase made by stepwise specific modification have been investigated. By a single step modification, one of the modified forms resulted in the conversion of native structure of glucose oxidase to molten globule like form [S. Hosseinkhani, B. Ranjbar, H. Naderi-Manesh, M. Nemat-Gorgani, FEBS Lett. 561 (2004) 213-216]. Chemical modification of lysine residues in glucose oxidase was carried out using different concentration of citraconic anhydride. Modification brought about changes in the tertiary structure with some degree of alteration in secondary structure. FTIR, far and near-UV CD spectropolarimetry, intrinsic and extrinsic fluorescence spectroscopy showed structural changes of glucose oxidase in a concentration dependent manner. This was supported by comparative study of secondary and tertiary structure.

Related Compounds

  • Citraconic anhydri...

Related Articles:

MALDI imaging mass spectrometry profiling of N-glycans in formalin-fixed paraffin embedded clinical tissue blocks and tissue microarrays.

2014-01-01

[PLoS ONE 9(9) , e106255, (2014)]

Polymeric micelles with citraconic amide as pH-sensitive bond in backbone for anticancer drug delivery.

2014-08-25

[Int. J. Pharm. 471(1-2) , 28-36, (2014)]

Pre-cultivation of adipose tissue-derived microvascular fragments in porous scaffolds does not improve their in vivo vascularisation potential.

2015-01-01

[Eur. Cell. Mater. 29 , 190-200; discussion 200-1, (2015)]

Experimental colitis in SIV-uninfected rhesus macaques recapitulates important features of pathogenic SIV infection.

2015-01-01

[Nat. Commun. 6 , 8020, (2015)]

Dual-targeting and pH/redox-responsive multi-layered nanocomplexes for smart co-delivery of doxorubicin and siRNA.

2015-08-01

[Biomaterials 60 , 42-52, (2015)]

More Articles...